741
HPLC CHROMATOGRAMS |
PHARMACEUTICAL
Ant ibiot ics, Ant i -Diabet ic, Cor t icosteroids
Anti-Diabetic Glyburide (Glybenclamide) on Ultra IBD
1
Peak List:
1. unknown
2. glyburide
Sample:
Inj.:
1µL
Conc.:
500µg/mL
Solvent:
water:methanol:acetonitrile
(1:1:2, v/v)
Column:
Ultra IBD
Cat.#:
9175565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
water with
0.1% (v/v) acetic acid:
acetonitrile (95:05, v/v)
Flow rate:
1.0mL/min.
Temp.:
27°C
Det.:
UV @ 254nm
2
0
1
2
3
4
5 min.
LC_0069
Peak List:
1. uracil (void marker)
2. triamcinolone
3. hydrocortisone
4. dexamethasone
5. corticosterone
6. deoxycorticosterone
Sample:
Inj.:
5µL
Conc.:
100µg/mL
Solvent:
mobile phase
Corticosteroids on Ultra C18
0
1
2
3
4
5
6 min.
LC_0108
3
2
1
4
5
6
Column:
Ultra C18
Cat.#:
9174565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
water:methanol
(30:70, v/v)
Flow:
1.0mL/min.
Temp.:
30°C
Det.:
UV @ 220nm
Corticosteroids on Allure® Biphenyl
LC_PH0330
Ret. Time
Peak List
(min.)
1. hydrocortisone
4.19
2. prednisone
4.79
3. cortisone
5.08
4. dexamethasone
6.37
5. corticosterone
9.01
6. cortisone acetate
15.75
7. desoxycorticosterone
25.94
Sample:
Inj.:
5µL
Conc.:
100µg/mL each component
Sample diluent: methanol
Column:
Allure
®
Biphenyl
Cat.#:
9166565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
water:acetonitrile, 60:40
Flow:
1mL/min.
Temp.:
ambient
Det.:
UV @ 254nm
Antibiotic Amoxicillin on Ultra IBD
Peak List:
1. amoxicillin
Sample:
Inj.:
5µL
Conc.:
1.5mg/mL
Solvent:
acetonitrile:water
(10:90, v/v)
Column:
Ultra IBD
Cat.#:
9175565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
10mM ammonium
formate, pH 2.5:
acetonitrile (95:5, v/v)
Flow rate:
1.2mL/min.
Temp.:
30°C
Det.:
UV @ 270nm
1
0
1
2
3
4
5
6 min.
LC_0095
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E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
