746
HPLC CHROMATOGRAMS |
BIOCHEMICAL/CLINICAL
Proteins, Oxytocin
Note excellent peak shapes and
resolution on the Viva column!
Protein Mix on Viva C18
Sample:
Inj.:
20µL
Conc.:
0.08mg/mL each protein
Sample diluent: 0.10% TFA in water / 0.10%
TFA in acetonitrile, 80:20, v/v
Sample temp.: 25°C
Columns:
Wide Pore C18
Dimensions:
150mm x 2.1mm
Particle size:
5µm
Pore size:
300Å
Conditions:
Mobile phase:
A: 0.10% TFA in water,
B: 0.10% TFA in acetonitrile,
20% B to 70% B in 30 min.
Flow:
0.20mL/min.
Temp.:
25ºC (or ambient)
Det.:
UV @ 214nm
Viva C18
(cat.# 9514562)
Peak List
Ret. Time (min.)
1. ribonuclease A
11.31
2. cytochrome c
14.65
3. holo-transferrin
16.32
4. apomyoglobin
20.34
Manufacturer A
Manufacturer B
Manufacturer C
Manufacturer D
LC_0324
free
literature
VivaWide Pore HPLC
Columns
Exceptional
Performance for
Peptides, Proteins, or
Other Large Molecules
Download your free
copy from
Flyer
lit. cat.# 59939
PEGylated Oxytocin on Viva C18 (LC/MS)
Sample:
oxytocin PEGylation reaction products
Inj.:
20µL
Conc.:
300pmoles/µL
Sample diluent: 0.1% formic acid in water (v:v)
Column:
Viva C18
Cat.#:
9514561
Dimensions:
150mm x 1.0mm
Particle size:
5µm
Pore size:
300Å
LC_PH0467
Conditions:
Mobile phase:
0.1% formic acid in water:0.1%formic acid in acetonitrile, 60:40
Flow:
0.1mL/min.
Temp.:
ambient
Det.:
Micromass Quattro II
Interface:
ESI
Ion mode:
positive
Temp.:
200°C
Capillary:
2.25kV
Cone:
40V
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
