748
HPLC CHROMATOGRAMS |
CLINICAL/FORENSIC
Opiates, THC
Opiates on Allure® PFP Propyl (LC/MS/MS)
Sample:
opiates
Inj.:
10µL
Conc.:
25ug/mL
Solvent:
methanol
Column:
Allure
®
PFP Propyl
Cat.#:
9169552
Dimensions:
50mm x 2.1mm
Particle size:
5µm
Pore size:
60Å
1. morphine
2. oxymorphone
3. hydromorphone
4. codeine
5. 6-monoacetylmorphine
6. oxycodone
7. hydrocodone
Conditions:
Instrument:
Shimadzu Prominence HPLC
Mobile phase: A: 0.1% formic acid in water
B: 0.1% formic acid in 80:20, methanol:acetonitrile
Time:
%B
0.0
10
3.00
50
6.00
50
6.10
10
8.10
Stop pumps
Flow:
0.40mL/min.
Temp.:
30°C
Det.:
Applied Biosystems/MDS Sciex API 3200™ MS/MS system
Ion source:
Electrospray, positive
IonSpray voltage: 5,500
Gas 1:
65psi (448kPa)
Gas 2:
45psi (310kPa)
Source temp.:
600°C
Mass Spectrometer Experiments:
Declustering
Collision
Compound
Q1
Q3
Potential (V)
Energy (V)
morphine
286
152
46
79
morphine
286
165
46
51
hydromorphone
286
185
46
41
hydromorphone
286
157
46
55
oxymorphone
302
227
36
37
oxymorphone
302
198
36
55
codeine
300
152
46
85
codeine
300
115
46
89
hydrocodone
300
199
46
39
hydrocodone
300
128
46
69
oxycodone
316
240
31
39
oxycodone
316
256
31
33
6-monoacetylmorphine 328
211
51
55
6-monoacetylmorphine 328
193
51
35
LC_PH0454
Column
Ultra II
®
Biphenyl (cat.# 9609853)
Dimensions:
50 mm x 3.0 mm ID
Particle Size:
2.2 µm
Pore Size:
100 Å
Temp.:
35 °C
Sample
Diluent:
Acetonitrile
Conc.:
50 ng/mL
Inj. Vol.:
20 µL
Mobile Phase
A: 0.1% formic acid in water
B: 0.1% formic acid in methanol
Time (min.)
%B
0
50
0.50
50
3.50
100
4.50
100
4.60
50
6.00
50
Flow:
0.7 mL/min.
Detector
Applied Biosystems/MDS Sciex LC/MS/MS
Model #:
3200 Q-Trap
Ion Source:
TurboIonSpray
®
Ion Mode:
ESI+
Ion Spray Voltage: 5 kV
Curtain Gas:
25 psi (172.4 kPa)
Gas 1:
50 psi (344.7 kPa)
Gas 2:
60 psi (413.7 kPa)
Source Temp.:
550 °C
Mode:
MRM
Dwell Time:
50 ms
Instrument
Applied Biosystems/MDS Sciex LC/MS/MS System
Peaks
MRM1 (Q1/Q3)
MRM2 (Q1/Q3)
1. Cannabidiol
315/123
315/193
2. THC-COOH-gluc
345/299
345/193
3. THC-COOH
345/299
345/193
4. Cannabinol
311/223
311/178
5.
Δ
9
-THC
315/193
315/123
THC and Metabolites on Ultra II® Biphenyl (LC/MS/MS)
LC_CF0512
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Mar 2011
