751
HPLC CHROMATOGRAMS |
CLINICAL/FORENSIC
Benzodiazepines
Benzodiazepines on Allure® PFP Propyl (LC/MS/MS)
Sample:
benzodiazepines
Inj.:
20µL
Conc.:
NA
Solvent:
NA
Column:
Allure
®
PFP Propyl
Cat.#:
9169552
Dimensions:
50mm x 2.1mm
Particle size: 5µm
Pore size:
60Å
Conditions:
Instrument:
Shimadzu Prominence HPLC
Mobile phase: A: 0.1% formic acid and 1mM
ammonium formate in water
B: 0.1% formic acid and 1mM
ammonium formate in acetonitrile
Time
Flow %B
(min.)
(µL/min.)
0.0
500
10
10.00
1000
90
15.00
1000
90
15.50
500
10
17.50
500
10
Flow:
see gradient table
Temp.:
40°C
Det.:
Applied Biosystems/MDS Sciex
API 3200™ MS/MS system
Ion Source:
Electrospray, positive
IonSpray
Voltage:
NA
Gas 1:
NA
Gas 2:
NA
Source
Temp.:
500°C
Data courtesy of: Applied Biosystems MDS Sciex
LC_PH0463
Analyze 27
benzodiazepines
in less than
10 minutes!
Compound Name
Retention Time Precursor Ion
MRM 1 MRM 2
DP
CE
CE
LOQ
(min.)
(amu)
(amu)
(amu)
(MRM 1) (MRM 2) (ng/mL)
7-aminonitrazepam
3.2
252.1
121.1
94.0
51
35
53
1.0
7-aminoclonazepam
3.3
286.1
121.0
222.2
46
41
35
0.5
7-aminoflunitrazepam
3.8
284.1
135.1
226.0
51
39
49
0.5
Bromazepam
3.8
316.0/318.0
182.1
182.1
51
45
45
5.0
α
-hydroxyalprazolam
4.1
325.1
297.2
204.9
51
31
59
2.0
α
-hydroxytriazolam
4.1
359.0
239.2
176.0
61
63
37
5.0
Oxazepam
4.2
287.0
241.1
268.9
41
27
19
10.0
Lorazepam
4.3
321.0/323.1
275.0
277.0
41
31
27
5.0
Estazolam
4.4
295.0
205.0
267.1
51
53
31
2.0
Zaleplon
4.4
306.2
236.3
264.2
56
35
27
0.5
2-hydroxyethylflurazepam 4.5
333.1
211.2
109.0
56
51
41
1.0
Desmethylflunitrazepam 4.5
300.1
254.2
198.2
56
35
51
2.0
Nitrazepam
4.6
282.0
236.1
180.2
71
35
51
2.0
Clonazepam
4.7
316.0
270.2
214.0
56
41
51
2.0
Desalkylflurazepam
4.7
289.1
140.1
226.1
71
41
39
2.0
Temazepam
4.7
301.1/303.1
255.1
257.2
35
30
30
5.0
Triazolam
4.7
343.0
238.9
314.9
61
53
37
1.0
Alprazolam
4.8
309.1
205.1
281.1
56
53
35
1.0
Lormetazepam
4.8
335.0/337.1
289.0
291.1
41
29
29
2.0
Clobazam
4.9
301.1
259.1
224.3
46
29
47
1.0
Flunitrazepam
5.0
314.0
268.1
239.1
56
35
49
1.0
Nordiazepam
5.0
271.1
140.2
164.9
46
37
35
2.0
Zolpiclone
5.4
389.1
244.8
217.0
16
25
41
1.0
D5-Diazepam
5.4
290.1
198.2
-
55
41
-
Diazepam
5.5
285.0
193.2
154.1
55
41
37
1.0
Chlordiazepoxide
6.0
300.1
227.1
283.2
36
31
21
5.0
Prazepam
6.1
325.1
271.1
140.0
81
31
53
2.0
Zolpidem
7.4
308.1
235.1
236.1
56
39
35
0.2
Midazolam
7.9
326.1
291.3
222.0
56
33
63
0.5
Flurazepam
8.5
388.2
315.1
317.1
36
27
27
0.1
Medazepam
9.0
271.0
91.1
207.3
46
41
39
2.0
D3-Doxepine
9.1
283.0
107.1
-
41
35
-
MRM Transitions, retention times,
and LOQ values.
Bar color indicates shared precursor ions.
Note compounds with shared precursor ions
are baseline resolved on the Allure
®
PFP
Propyl column, as shown by retention time
comparison.
Data courtesy of Applied Biosystems MDS Sciex.
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
