742
HPLC CHROMATOGRAMS |
PHARMACEUTICAL
Steroids
Endogenous Steroids on Allure® Biphenyl
LC_PH0334
Ret. Time
Peak List
(min.)
1.
β
-estradiol
3.76
2. testosterone
5.08
3. progesterone
14.05
Sample:
Inj.:
5µL
Conc.:
100µg/mL each component
Sample diluent: methanol
Column:
Allure
®
Biphenyl
Cat.#:
9166565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
water:acetonitrile, 40:60
Flow:
1mL/min.
Temp.:
ambient
Det.:
UV @ 220nm
Contraceptive Hormones on Allure® Biphenyl
LC_PH0328
Ret. Time
Peak List
(min.)
1.
β
-estradiol
3.80
2. ethynyl estradiol
4.45
3. norethindrone
6.15
4. norgestrel
8.41
Sample:
Inj.:
5µL
Conc.:
100µg/mL each
component
Sample diluent: methanol
Column:
Allure
®
Biphenyl
Cat.#:
9166565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase: water:acetonitrile,
40:60
Flow:
1mL/min.
Temp.:
ambient
Det.:
UV @ 220nm
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Peak List:
1. estriol
2. 17
β
-estradiol
3. 17
α
-estradiol
4. ethynyl estradiol
5. testosterone
6. estrone
7. norethindrone
Sample:
Inj.:
1µL
Conc.:
100µg/mL each component
Sample diluent: water:methanol (50:50)
Column:
1.9µm Pinnacle
®
DB Biphenyl
Cat.#:
9409252
Dimensions:
50mm x 2.1mm
Particle size:
1.9µm
Pore size:
140Å
Conditions:
Mobile phase:
A: water
B: methanol
Time(min)
%B
0
30
1
30
3
70
Flow:
0.8 mL/min.
Temp.:
30ºC
Det.:
UV @ 220nm
LC_PH0453
Hormones on 1.9μm Pinnacle® DB Biphenyl
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
