734
HPLC CHROMATOGRAMS |
FOODS & FLAVORS
Vi tamins & Dietar y Supplements
Water Soluble B Vitamins
Ultra II® Aqueous C18
Sample:
Inj.:
1µL
Conc.:
100µg/mL each component
Sample diluent: water:methanol (70:30)
Column:
Ultra II
®
Aqueous C18
Cat.#:
9608565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Instrument:
Shimadzu Prominence UFLC
XR
Mobile phase:
A. 10mM potassium phosphate (pH 2.5)
B. acetonitrile
Time (min.)
%B
0
2
1.5
2
5
75
Flow:
1.5mL/min.
Temp.:
40°C
Det.:
UV @ 210nm
Pressure:
105bar
Peak List
Ret. Time (min.)
1. vitamin B
3
2.472
2. vitamin B
6
2.764
3. vitamin B
5
3.804
4. vitamin B
9
4.126
5. vitamin B
12
4.261
6. vitamin B
2
4.329
7. vitamin B
7
4.472
LC_PH0503
NEW!
Vitamin C
Ultra II® Aqueous C18
Sample:
Inj.:
2µL
Conc.:
100µg/mL
Sample diluent: water
Column:
Ultra II
®
Aqueous C18
Cat.#:
9608565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Instrument:
Shimadzu Prominence UFLC
XR
Mobile phase:
100% 10mM potassium phosphate (pH2.5)
Flow:
1.5mL/min.
Temp.:
40°C
Det.:
UV @ 242nm
Pressure:
95bar
Peak List
Ret. Time (min.)
1. ascorbic acid (vitamin C)
1.759
LC_PH0504
NEW!
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
