743
HPLC CHROMATOGRAMS |
PHARMACEUTICAL
Lactulose Concentrate, Xanthines, Admixed Drugs
Admixtures: Expectorant/Antitussive Guaifenesin and
Narcotic Analgesic Codeine on Ultra Phenyl
1
Peak List:
Conc. (µg/mL)
1. codeine
825
2. guaifenesin
375
Sample:
Inj.:
5µL
Solvent:
methanol:water (50:50)
Column:
Ultra Phenyl
Cat.#:
9105565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
10mM ammonium
formate, pH 2.5:
acetonitrile
(80:20, v/v)
Flow rate:
1.2mL/min.
Temp.:
25°C
Det.:
UV @ 280nm
0
1
2
3
4
5
6 min.
LC_0088
2
Peak List:
Conc.
Ret. Time RRT Tailing Resolution Inj. % RSD
(µg/mL)
(min.)
(n = 5)
1. acetaminophen
0.76
3.304
1.0
1.1
— 1.1
2. hydrocodone bitartrate 0.0076 6.664
2.0
1.1
17.0
0.9
Sample:
Inj.:
25µL
Sample:
500mg acetaminophen with
5mg hydrocodone bitartrate (tablet)
Solvent:
mobile phase
LC_0212
Admixtures: Hydrocodone Bitartrate/Acetaminophen
by USP 25 on Ultra C18
Column:
Ultra C18
Cat.#:
9174575
Dimensions:
250mm x 4.6mm
Particle size: 5µm
Pore size:
100Å
Conditions:
Mobile phase:
6.8g potassium
monobasic with 0.2mL
TEA per liter:
acetonitrile
(85:15, v/v)
Flow:
1.5mL/min.
Temp.:
27°C
Det.:
UV @ 210nm
(hydrocodone
bitartrate) and 295nm
(acetaminophen).
Peak List:
Conc. (mg/mL)
Ret. Time (min.)
RRT Res.
Inj. % RSD
(n = 5)
1. fructose
0.4
4.6
0.44
— —
2. galactose
6.4
6.1
0.58
— —
3. epi-lactose unknown
10.0
0.95
— —
4. lactulose
40
10.5
1.00
1.1
0.3%
5. lactose
4.8
12.7
1.21
2.9
—
Sample:
Inj.: 20µL resolution standard
Solvent: acetonitrile:water (50:50)
LC_0222
Laxative Lactulose Concentrate by USP 25: Resolution
Solution on Pinnacle® II Amino
Column:
Pinnacle
®
II Amino
Cat.#:
9217365
Dimensions:
150mm x 4.6mm
Particle size:
3µm
Pore size:
110Å
Conditions:
Mobile phase:
10mM sodium phosphate
monobasic in water, pH 4.8:
acetonitrile (22:78, v/v)
Flow:
1.3mL/min.
Temp.:
40°C
Det.:
refractive index @ 40°C
0
1
2
3
4
5
6
7
min.
1
2
3
4
LC_0241
Xanthines on Pinnacle® DB C18
Sample:
Inj.:
2µL
Solvent:
mobile phase
Column:
Pinnacle
®
DB C18
Cat.#:
9414565
Dimensions: 150mm x 4.6mm
Particle size: 5µm
Pore size:
140Å
Conditions:
Mobile phase: 20mM KH
2
PO
4
,
pH 3.0:
acetonitrile
(90:10 v/v)
Flow:
1.0mL/min.
Temp.:
27°C
Det.:
UV @ 254nm
Peak List:
Conc. (µg/mL)
1. theobromine
225
2. theophylline
418
3.
β
-hydroxyethyltheophylline
418
4. caffeine
400
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
