Pain Management Drugs in Urine on Ultra II® Biphenyl (LC/MS/MS)
Peaks
RT (min.) MRM1 MRM2
1. Acetaminophen 1.18 152/110 152/65
2. Morphine
1.79 286/157 286/181
3. Codeine
3.21 300/165 300/215
4. Oxycodone
3.46 316/298 316/241
5. Hydrocodone
3.60 300/199 300/171
6. Fentanyl
6.61 337/132 337/216
7. Buprenorphine 6.66 468/187 468/84
8. Lorazepam
6.79 321/275 321/229
9. Diazepam
8.06 285/154 285/193
10. Methadone
8.21 310/77 310/223
Column
Ultra II
®
Biphenyl (cat.# 9609352)
Dimensions:
50 mm x 2.1 mm ID
Particle Size:
3 µm
Pore Size:
100 Å
Temp.:
40 °C
Sample
Conc.:
5 ng/mL in 1:10 urine:mobile phase
Inj. Vol.:
5 µL
Mobile Phase
A: 0.1% formic acid in water
B: 0.1% formic acid in methanol
Time (min.) Flow (mL/min.)
%A %B
0
0.5
90 10
10
0.5
0 100
10.1
0.5
90 10
12
0.5
90 10
Detector
Applied Biosystems/MDS Sciex LC/MS/MS
Model #:
API-5000™
Ion Source:
TurboIonSpray
®
Ion Mode:
ESI+
Curtain Gas:
25 psi (172.4 kPa)
Gas 1:
60 psi (413.7 kPa)
Gas 2:
40 psi (275.8 kPa)
Source Temp.:
550 °C
Source Voltage:
2000 V
Mode:
MRM
Dwell Time:
50 ms
Instrument
Applied Biosystems/MDS Sciex
LC/MS/MS System
Acknowledgement Special thanks to Applied Biosystems
for providing instrument time.
LC_CF0516
Recommended for opiates.
NEW!
747
HPLC CHROMATOGRAMS |
CLINICAL/FORENSIC
Pain Management Drugs
For more information on Biphenyl columns, visit
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
