750
HPLC CHROMATOGRAMS |
CLINICAL/FORENSIC
Amphetamines, Cocaine
Amphetamines on Ultra II® Biphenyl (LC/MS)
Column
Ultra II
®
Biphenyl (cat.# 9609552)
Dimensions:
50 mm x 2.1 mm ID
Particle Size:
5 µm
Pore Size:
100 Å
Temp.:
30 °C
Sample
Diluent:
mobile phase
Conc.:
2 µg/mL
Inj. Vol.:
5 µL
Mobile Phase
A: 0.1% formic acid in water
B: 0.1% formic acid in methanol
Time (min.) Flow (mL/min.) %A %B
0
0.3
80 20
10
0.3
5 95
10.1
0.3
80 20
12.0
0.3
80 20
Detector
Shimadzu 2010EV
Acquisition Type: Scan
Scan Speed:
2000 amu/sec.
Heat Block:
200 °C
Interface:
ESI+
Interface Temp.: 250 °C
Scan Range:
100 - 250 amu
Event Time:
0.1 sec.
Instrument
Shimadzu UFLC
XR
Notes Data were collected in scan mode. An extracted ion chromatogram is shown.
LC_CF0518
Peaks
RT (min.)
m/z
1. Amphetamine
2.812
135.95
2. Methamphetamine 3.432
149.95
3. MDA
3.537
179.95
4. MDMA
4.092
193.95
5. MDEA
4.679
207.95
NEW!
Cocaine and Ecgonine Methyl Ester on
Allure® PFP Propyl (LC/MS/MS)
Peak List:
1. EME (ecgonine methyl ester)
2. COC (cocaine)
Sample:
Inj.:
10µL
Conc.:
1µg/mL
Solvent:
water
Temp.:
4°C
Column:
Allure
®
PFP Propyl
Cat.#:
9169532
Dimensions: 30mm x 2.1mm
Particle size: 5µm
Pore size: 60Å
Data Courtesy of Shane Needham, Pfizer Inc.
LC_0126
XIC of +MRM (3 pairs) for
304.0 / 182.0 amu
2.35e3 cps
2
0
0.5 1.0 1.5 2.0 2.5 3.0
3.5 4.0 4.5
min.
XIC of +MRM (3 pairs) for
200.0 / 182.0 amu
1.30e3 cps
1
0
0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5
min.
Conditions:
Mobile phase:
5mM ammonium
formate, pH 3.0:
acetonitrile (10:90, v/v)
Flow:
0.6mL/min.
Column temp.:
ambient
Det.:
Applied Biosystems/MDS SCIEX
API 3200™ MS/MS system
Interface:
turbo ion spray, ESI
Interface temp.:
150°C
Ion mode:
positive
ESI probe voltage:
5,000V
Orifice:
+ 71V
Ring:
+ 265V
Collision gas:
nitrogen
Collision gas pressure:
2.2 mTorr
Collision gas energy:
28 eV (COC)
26 eV (EME)
Electron multiplier:
2,100 volts
Auxilary gas flow:
7,000cc/min.
Nebulizer gas setting:
15lb/in.
2
Curtain gas setting:
12lb/in.
2
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For more information on Biphenyl columns, visit
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
