740
HPLC CHROMATOGRAMS |
PHARMACEUTICAL
Ant ibiot ics
Antibiotic Cephalexin on Ultra IBD
1
Peak List:
1. cephalexin
2. cephradine
Sample:
Inj.:
10µL
Conc.:
500µg/mL
Solvent:
acetonitrile:water
(10:90, v/v)
Column:
Ultra IBD
Cat.#:
9175565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
10mM ammonium
formate, pH 2.5:
acetonitrile
(90:10, v/v)
Flow rate:
1.2mL/min.
Temp.:
30°C
Det.:
UV @ 270nm
2
0
2
4
6
8
10 min.
LC_0094
Peak List:
1. penicillin V
Sample:
Inj.:
2.5µL
Conc.:
1.2mg/mL
Solvent:
acetonitrile:water
(10:90,v/v)
Column:
Ultra IBD
Cat.#:
9175565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
10mM ammonium
formate, pH 2.5:
acetonitrile (95:5, v/v)
Flow rate:
1.2mL/min.
Temp.:
30°C
Det.:
UV @ 270nm
Antibiotic Penicillin V on Ultra IBD
1
0
1
2
3
4
5
6
7
8 min.
LC_0096
Antibiotic Cephaloridine on Ultra IBD
(Reversed Phase)
Peak List:
1. cephaloridine
Sample:
Inj.:
5µL
Conc.:
1mg/mL
Solvent:
acetonitrile:water
(50:50, v/v)
Column:
Ultra IBD
Cat.#:
9175565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
acetonitrile:
20mM ammonium phosphate,
pH 4.0 (20:80, v/v)
Flow:
1.2mL/min.
Temp.:
27°C
Det.:
UV @ 254nm
LC_0102
1
0
1
2
3
4
5
6
7
8
min.
Tetracycline Mix on Allure® Biphenyl
LC_PH0347
Ret. Time
Peak List
(min.)
1. oxytetracycline
4.59
2. degradation peak
5.19
3. tetracycline
6.32
4. degradation peak
11.16
5. chlortetracycline
15.81
6. doxycycline
19.66
Sample:
Inj.:
30µL
Conc.:
100µg/mL each component
Sample diluent: methanol
Column:
Allure
®
Biphenyl
Cat.#:
9166565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
20mM ammonium phosphate
(pH 2.5):acetonitrile, 80:20
Flow:
1mL/min.
Temp.:
ambient
Det.:
UV @ 254nm
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
