731
HPLC CHROMATOGRAMS |
FOODS & FLAVORS
Vi tamins & Dietar y Supplements
LC_CF0492
Sample:
Inj.:
20µL
Conc.:
extracted serum sample
Column:
Ultra Aqueous C18
Cat.#:
9178352
Dimensions:
50mm x 2.1mm
Particle size:
3µm
Pore size:
100Å
Conditions:
Instrument:
Shimadzu Prominence
®
UFLC
XR
Mobile phase:
A: 0.1% formic acid in water
B: 0.1% formic acid in methanol
Time (min.) %B
0.00
50
2.5
100
3.5
100
3.6
50
5.00
50
Flow:
700µL/min.
Temp.:
40°C
Det.:
Applied Biosystems 3200 QTRAP
™
LC/MS/MS system
Ion source:
TurboIonSpray
®
, APCI+
Mode:
MRM
Dwell time:
100msec.
Declustering
Ret. Time
Compound
Q1
Q3 potential (V)
(min.)
d6-25-OH D
3
(IS)
389.3
211.2
68
3.00
25-OH D
3
383.3
211.2
68
3.00
25-OH D
2
395.3
229.2
55
3.04
25-OH D
3
383.3
229.2
68
3.00
25-OH D
2
395.3
269.2
55
3.04
25-OH D
2
395.3
119.0
55
3.04
Vitamin D on
Ultra Aqueous C18 (LC/MS/MS)
NEW!
for
more
info
Visit
to view our "
5 Minute Analysis
of Vitamin D in Serum by LC/MS/MS
" article.
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
