722
HPLC CHROMATOGRAMS |
FOODS & FLAVORS
Food Contaminants
Sample:
Inj.:
20µL, mixture of Sudan I, Sudan II, Sudan III, Sudan IV
Conc.:
20µg/mL each component
Sample diluent: methanol
Column:
Ultra Aqueous C18
Cat.#:
9178565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
methanol:water, 97:3
Flow:
1mL/min.
Temp.:
ambient
Det.:
UV @ 476/493/512/357nm (left) or UV @ 488/520nm (right)
Sudan Dyes on Ultra Aqueous C18
1. Sudan I
2. Sudan II
3. Sudan III
4. Sudan IV
LC_FF0326
LC_FF0327
Fixed Dual Wavelength
Detector
wavelength change
Photodiode Array
Detector
Monitor Sudan I, II, III, and IV
in a single, isocratic analysis!
Pesticides on Pinnacle® DB Aqueous C18 (LC/MS/MS, ESI-)
LC_FF0479B
Sample:
multicomponent pesticide
standard
Inj.:
5µL
Conc.:
33.3ppb each pesticide
Sample diluent: acetonitrile
Column:
Pinnacle
®
DB Aqueous C18
Cat.#:
9418252
Dimensions:
50mm x 2.1mm
Particle size:
1.9µm
Pore size:
140Å
Conditions:
Mobile phase: A: 10 mM NH
4
OAc in water
B: 10 mM NH
4
OAc in methanol
Time (min.)
%B
0.0
10
1.0
10
8.0
90
10.0
90
11.0
10
Flow:
600µL/min
Temp.:
35°C
Instrument:
Shimadzu Prominence
®
UFLCXR
Det.:
Applied Biosystems 4000 QTRAP
®
LC/MS/MS system
Ion Source: TurboIonSpray
®
, ESI-
IonSpray Voltage, ESI voltage: -4.2kV (ESI-)
Gas 1: 40psi
Gas 2: 60psi
Source Temp.: 500°C; max pressure ~7,200psi
Peak List:
Compound
Polarity Mol. Wt.
RT (min.)
Transition 1
bentazone
negative 240.28
0.77
239.0
→
131.8
bromoxynil
negative 276.91
1.32
275.9
→
80.8
Ioxynil
negative 370.92
2.46
369.8
→
126.7
fludioxinil
negative 248.19
5.34
247.0
→
179.9
boscalid
negative 343.21
5.36
343.0
→
307
prothioconazole negative 344.26
5.41
341.9
→
305.8
fipronil
negative 437.15
5.56
434.9
→
329.8
noviflumuron negative 529.1
7.08
527.1
→
343.8
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Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
