715
HPLC CHROMATOGRAMS |
ENVIRONMENTAL
Herbicides
Peak List:
Peak time (min.)
1. diquat
7.5
2. paraquat
8.8
Sample:
Inj.:
20µL
Conc.:
20ppm each component (above);
20µg/mL, 40µg/mL, and 100µg/mL
each component at 308nm (left)
and at 257nm (right)
Sample diluent: water
Column:
Ultra Quat
Cat.#:
9181565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Conditions:
Mobile phase:
Ultra Quat Reagent Solution (cat.# 32441):
acetonitrile, 95:5 (v/v)
Flow:
1.0 mL/min.
Temp.:
27°C
Det.:
UV @ 257nm (paraquat)
UV @ 308nm (diquat)
Paraquat and Diquat on Ultra Quat
min.
1
2
LC_0321
min.
diquat
308nm
paraquat
257nm
100µg/mL
40µg/mL
20µg/mL
100µg/mL
40µg/mL
20µg/mL
Paraquat and Diquat on Ultra Quat
Sample:
Inj.:
10µL
Conc.:
5µg/mL each component
Sample diluent: DI water
Sample temp.: ambient
Column:
Ultra Quat
Cat.#:
5181352
Dimensions:
50mm x 2.1mm
Particle size:
3µm
Pore size:
100Å
Conditions:
Mobile phase:
10mM heptafluorobutyric acid:acetonitrile, 95:5 (v/v)
Flow:
0.3mL/min.
Temp.:
ambient
Det.:
Applied Biosystems/MDS SCIEX API 3200™
MS/MS system
Interface:
electrospray
Ion Mode:
positive
Temp.:
600°C
Ion Spray
voltage:
5,500V
Collision exit
potential:
3V
Curtain Gas:
15psi (103kPa)
Gas supply 1:
70psi (483kPa)
Gas supply 2:
60psi (414kPa)
Quantitation:
(MRM)
Q1/Q3:
unit resolution
Dwell time:
200 ms
Precursor Ion Fragment Ion DP Collision Energy
(amu)
(amu)
(V)
(eV)
diquat, 183+ 157+ 30
30
paraquat, 93 (2+)
171+ 20
20
Peak List
1. diquat
2. paraquat
*Data courtesy of Houssain El Aribi, Ph.D., LC/MS Product and Application Specialist, MDS SCIEX,
71 Four Valley Drive, Concord, Ontario, Canada, L4K 4V8
LC_EV0383
paraquat dichloride [CAS# 1910-42-5]
diquat dibromide
[CAS # 85-00-7]
Fast, sensitive
LC/MS/MS analysis!
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
