721
HPLC CHROMATOGRAMS |
FOODS & FLAVORS
Food Contaminants
Sample:
multicomponent pesticide standard
Inj.:
10µL
Conc.:
1ppb each pesticide
Sample diluent:
acetonitrile
Column:
Ultra Aqueous C18
Cat. #:
9178312
Dimensions:
100mm x 2.1mm
Particle size:
3µm
Pore size:
100Å
Conditions:
Instrument:
Shimadzu Prominence
®
UFLC
XR
Mobile phase:
A: 10 mM NH
4
OAc in water
B: 10 mM NH
4
OAc in methanol
Time (min.)
%B
0.0
20
8.0
90
12.0
100
14.8
100
14.9
20
Flow:
500µL/min
Temp.:
35°C
Det.:
Applied Biosystems 4000 QTRAP
®
LC/MS/MS system
Ion Source:
TurboIonSpray
®
A & C: ESI+
B: ESI-
IonSpray Voltage: 5kV (ESI+), -4.2kV (ESI-)
Gas 1:
50psi
Gas 2:
60psi
Source Temp.:
600°C
Pesticides on
Ultra Aqueous C18 (LC/MS/MS)
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
11.0
12.0
13.0
14.0
15.0
Time (min.)
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
11.0
12.0
13.0
14.0
15.0
Time,min
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
11.0
12.0
13.0
14.0
Time (min.)
0.0
A: Pesticides in positive ion mode
B: Pesticides in negative ion mode
C: Improved retention of difficult
pesticides in positive ion mode
LC_FF0490A
LC_FF0490
LC_FF0490B
methamidophos
acephate
omethoate
propamocarb
NEW!
Visit
to
view our "
Comprehensive Pesticide
Residue Analysis by LC/MS/MS Using an
Ultra Aqueous C18 Column
" article
(contains compound identifications
and MS details).
for
more
info
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
