725
HPLC CHROMATOGRAMS |
FOODS & FLAVORS
Drug Residues
Sample:
Inj.:
3µL
Conc.:
25µg/mL each component
Sample diluent: methanol
Column:
Ultra II
®
Biphenyl
Cat.#:
9609853
Dimensions:
50mm x 3.0mm
Particle size:
2.2µm
Pore size:
100Å
Conditions:
Instrument:
Shimadzu Prominence UFLC
XR
Mobile phase:
0.1% formic acid
in water:methanol (75:25)
Time (min.) %B
0
25
3.5
65
Flow:
1.0mL/min.
Temp.:
30°C
Det.:
UV @ 254nm
Peak List
Ret. Time (min.)
1. sulfadiazine
1.496
2 sulfathiazole
1.663
3. sulfamerazine
1.997
4. sulfamethoxazole
2.381
5. sulfamethazine
2.523
6. sulfachlorpyridizine
2.651
7. sulfamethoxine
3.228
Sulfa Drugs
Ultra II® Biphenyl
LC_PH0489
NEW!
Sample:
milk spiked at 200ppb with sulfonamides, extracted with Q-sep™ Q110 QuEChERS
extraction tube (cat.# 26213), and cleaned up with Q-sep™ Q211 QuEChERS dSPE
clean-up tube (cat.# 26216), then concentrated.
Inj.:
10µL
Conc.:
4µg/mL
Sample diluent: 0.2% formic acid
Column:
Pinnacle
®
DB Biphenyl
Cat. #:
9409312
Dimensions:
100mm x 2.1mm
Particle size:
3µm
Pore size:
140Å
Conditions:
Instrument:
Agilent 1100 with LECO Unique
®
LC-TOFMS
Mobile phase:
A: 0.2% formic acid
B: methanol:acetonitrile (80:20), with 0.2% formic acid
Time (min.)
%B
0
15
20
100
30
100
Flow:
1mL/min. with preinjection split delivering 0.2mL/min. to column
Temp.:
ambient
Det.:
LECO Unique
®
LC-TOFMS
Interface:
ESI
Ion mode:
positive
Temp.:
120°C
Capillary:
3.8kV
Cone:
50V
Desolvation gas: 4L/min.
Collected:
200-1,000 m/z
Compound
[M+H]
+
1. sulfadiazine
251
2. sulfathiazole
256
3. sulfamerazine
265
4. sulfamethazine
279
5. sulfamethoxypyridazine
281
6. sulfamethoxazole
254
7. sulfachloropyridazine
285
8. sulfadimethoxine
311
9. sulfaquinoxaline
301
Sulfonamides in Milk on Pinnacle® DB Biphenyl (LC-TOFMS)
LC_FF0502
NEW!
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
