733
HPLC CHROMATOGRAMS |
FOODS & FLAVORS
Vi tamins & Dietar y Supplements
min.
0.00
0.25
0.50
0.75
1.00
1.25
1.50
1.75
2.00
2.25
2.50
Fat Soluble Vitamins
Ultra II® C18
Peak List
Ret. Time (min.)
1. vitamin K2
0.905
2. vitamin D2
1.224
3. vitamin D3
1.305
4. vitamin E acetate
1.828
5. vitamin K1
2.169
Sample:
Inj.:
1µL
Conc.:
100µg/mL each component
Sample diluent: acetone
Column:
Ultra II
®
C18
Cat.#:
9604853
Dimensions:
50mm x 3.0mm
Particle size:
2.2µm
Pore size:
100Å
Conditions:
Instrument:
Shimadzu Prominence UFLCXR
Mobile phase:
acetonitrile:methanol (85:15)
Flow:
1.5mL/min.
Temp.:
ambient
Det.:
UV @ 230nm
1
2
3
4
5
LC_PH0492
NEW!
Vitamins B1, B2, B3, and B6
Ultra C8
Peak List
Ret. Time (min.)
1. vitamin B
3
(nicotinamide)
2.732
2. vitamin B
6
(pyridoxine)
5.615
3. vitamin B
2
(riboflavin)
8.719
4. vitamin B
1
(thiamine)
19.004
Sample:
Inj.:
5µL
Conc.:
50µg/mL each component
Sample diluent: water:methanol (80:20)
Column:
Ultra C8
Cat.#:
9103575
Dimensions:
250mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Instrument:
Shimadzu Prominence UFLC
XR
Mobile phase:
0.008M sodium 1-hexanesulfonate:methanol:glacial acetic acid:
triethylamine (817.5:175:7.5:0.2)
Flow:
2.0mL/min.
Temp.:
50°C
Det.:
UV @ 270nm
LC_PH0497
NEW!
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
