727
HPLC CHROMATOGRAMS |
FOODS & FLAVORS
Preser vat ives, Sugars
LC_0198
1
2
3
4
0
2
4
6
8
10
12
14
16
18min.
Phenolic Antioxidants on Pinnacle® II C18
Peak List:
Conc. (ppm)
1. propyl gallate
168
2. TBHQ
182
3. 2-BHA + 3-BHA 197
4. BHT
193
Sample:
Inj.:
10µL
Solvent:
methanol
Column:
Pinnacle
®
II C18
Cat.#:
9214565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
110Å
Conditions:
Mobile phase: A: 1% acetic acid
B: methanol
Time B
(min.) (%)
0 50
4 50
10 90
25 90
26 50
Flow:
1.0mL/min.
Temp.:
30°C
Det.:
UV @ 280nm
Peak List:
Conc. (mg/mL)
1. fructose
2.0
2. glucose
2.1
3. sucrose
4.0
4. maltose
4.5
5. lactose
4.4
LC_0223
Sugars on Pinnacle® II Amino (3μm)
Column:
Pinnacle
®
II Amino
Cat.#:
9217365
Dimensions:
150mm x 4.6mm
Particle size:
3µm
Pore size:
110Å
Conditions:
Mobile phase: water:acetonitrile (25:75, v/v)
Flow:
1.5mL/min.
Temp.:
35°C
Det.:
refractive index @ 35°C
Sample:
Inj.:
5µL
Solvent:
mobile phase
LC_0252
Vanilla Bean Extract on Pinnacle® DB C18
(Fast LC)
Peak List
Ret. Time (min.)
1. vanillin
2.00
Column:
Pinnacle
®
DB C18
Cat.#:
9414555
Dimensions:
50mm x 4.6mm
Particle size:
5µm
Pore size:
140Å
Conditions:
Mobile phase:
0.2% v/v phosphoric acid in
water:methanol (80:20 v/v)
Flow:
2mL/min.
Temp.:
27°C
Det.:
UV @ 254nm
Sample:
Inj.:
5µL
Conc.:
20% v/v
commercial extract in water
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Mar 2011
