732
HPLC CHROMATOGRAMS |
FOODS & FLAVORS
Vi tamins & Dietar y Supplements
Peak List:
Conc. (mg/mL)
1. solvent front
n/a
2. menadione (vitamin K3)
0.45
3. all-
trans
-retinol (vitamin A)
0.34
4. vitamin D3
0.4
5. unknown
n/a
6.
α
tocopherol (vitamin E)
2.4
7.
α
tocopherol acetate
(vitamin E acetate)
2.4
8. unknown
n/a
9. phylloquinone (vitamin K1)
0.84
Vitamins (Fat Soluble) on Ultra C18
Column:
Ultra C18
Cat.#:
9174575
Dimensions:
250mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase: acetonitrile:methanol
(90:10, v/v)
Time (min.) Flow (mL/min.)
1.00
1.00
5.00
1.00
5.01
2.00
50.0
2.00
Temp.:
30°C
Det.:
UV @ 280nm
1
2
3
4
5
6
7
8
9
0
10
20
30
40 min.
LC_0144
Sample:
Solvent:
diethyl ether
Sample:
Inj.:
1.0µL
Solvent:
mobile phase
Column:
Ultra IBD
Cat.#:
9175565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
50mM potassium
phosphate, pH 2.5
Flow:
1.0mL/min.
Temp.:
ambient
Det.:
UV @ 254nm
LC_0227
Vitamins Thiamin (Vitamin B1) and Ascorbic Acid
(Vitamin C) on Ultra IBD
Peak List:
Conc. (mg/mL)
1. thiamin
2.5
2. ascorbic acid
2.5
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Mar 2011
