719
HPLC CHROMATOGRAMS |
FOODS & FLAVORS
Food Contaminants
Carbamate Pesticides on Ultra II® Carbamate (LC/MS/MS)
Time (min.)
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
Peaks
RT (min.)
Transition 1
Transition 2
1. Aldicarb sulfone
1.9
223.1
→
86.1 223.1
→
148.0
2. Aldicarb sulfoxide
2.2
207.1
→
132.1 207.1
→
89.1
3. Oxamyl
2.4
237.1
→
72.1 237.1
→
90.1
4. Methomyl
2.6
163.1
→
88.1 163.1
→
106.0
5. 3-Hydroxycarbofuran
3.5
238.1
→
163.1 238.1
→
181.2
6. Aldicarb
4.5
208.1
→
116.0 208.1
→
88.9
7. Propoxur
5.1
210.1
→
111.0 210.1
→
168.1
8. Carbofuran
5.3
222.1
→
165.2 222.1
→
123.0
9. Carbaryl
5.8
202.1
→
145.1 202.1
→
127.1
10. Methiocarb
7.0
226.1
→
169.2 226.1
→
121.0
1
2
3
4
5
6
7
8
9
10
LC_EV0510
Column
Ultra II
®
Carbamate (cat.# 9611312)
Dimensions:
100 mm x 2.1 mm ID
Particle Size:
3 µm
Pore Size:
100 Å
Temp.:
35 °C
Sample
531.1 Carbamate Pesticide Calibration Mixture
(cat.# 32273)
Diluent:
acetonitrile
Conc.:
100 ppb each pesticide
Inj. Vol.:
5 µL
Mobile Phase
A:
10 mM NH
4
OAc in water
B:
10 mM NH
4
OAc in methanol
Time (min.)
%B
0
10
1
10
8
90
10
90
11
10
Flow:
0.5 mL/min.
Instrument
Applied Biosystems/MDS Sciex LC/MS/MS System
Detector
Applied Biosystems/MDS Sciex LC/MS/MS
Model #:
4000 QTRAP™ LC/MS/MS system
Ion Source:
TurboIonSpray
®
Ion Mode:
ESI+
Ion Spray Voltage: 5 kV
Gas 1:
40 psi (275.8 kPa)
Gas 2:
60 psi (413.7 kPa)
Source Temp.:
500 °C
NEW!
Carbamates in Orange Oil on Ultra Carbamate (LC-TOFMS)
Peak List:
1. aldicarb sulfone
2. aldicarb sulfoxide
3. oxamyl
4. methomyl
5. 3-hydroxycarbofuran
6. aldicarb
7. propoxur
8. carbofuran
9. carbaryl
10. methiocarb
11. BDMC (IS)
Sample:
531.1 Carbamate Pesticide Calibration Mix (cat.# 32273) and
Internal Standard 4-bromo-3,5-dimethylphenyl-N-methylcarbamate
(cat.# 32274) 50:50 spiked into unprocessed orange oil at 10ppm
Inj.:
3µL
Conc.:
10ppm
Sample diluent:
methanol
Matrix:
orange oil
Column:
Ultra Carbamate
Cat. #:
9177352
Dimensions:
50mm x 2.1mm
Particle size:
3µm
Pore size:
100Å
Conditions:
Mobile phase:
A: 2mM ammonium acetate:methanol, 90:10
B: 2mM ammonium acetate:methanol, 10:90
Time (min.)
%B
0
20
20
100
25
100
Flow:
200µL/min.
Temp.:
ambient
Det.:
LECO Unique
®
LC-TOFMS
Interface:
ESI
Ion mode:
Positive
Temp.:
130°C
Nebulizer pressure:
100kPa
Desolvation gas (N2): 4,000cc/min.
Interface temperature: 120°C
Nozzle:
(+) 62V
Capillary:
(+) 2.75kV
LC_FF0472
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
