713
HPLC CHROMATOGRAMS |
ENVIRONMENTAL
Herbicides
Phenoxyacid Herbicides on Ultra II® Aqueous C18 (LC/MS/MS)
Peaks
RT (min.)
MRM 1
MRM 2
1. Dicamba
1.14 219.0
→
175.0 221.0
→
177.0
2. Bentazon
1.32 239.0
→
132.0 239.0
→
175.0
3. 2,4-D
3.10 218.9
→
161.0 220.9
→
163.0
4. 3,5-DCBA
3.10 189.0
→
144.8 189.0
→
59.0
5. 4-Nitrophenol
3.10 138.1
→
108.1 138.1
→
46.0
6. MCPA
3.16 199.0
→
141.0 201.0
→
143.0
7. Dichlorprop
4.02 233.0
→
161.0 235.0
→
163.0
8. 2,4,5-T
4.46 252.9
→
194.9 254.9
→
196.9
9. Dinoseb
5.03 239.0
→
133.9 239.0
→
193.8
10. 2,4-DB
5.18 247.0
→
161.0 249.0
→
163.0
11. 2,4,5-TP
5.20 266.9
→
195.0 266.9
→
159.0
12. Acifluorfen
5.67 359.9
→
316.1 359.9
→
195.0
13. Pentachlorophenol
6.20 264.8
→
35.0
264.8
→
37.0
Column
Ultra II
®
Aqueous C18 (cat.# 9608312)
Dimensions:
100 mm x 2.1 mm ID
Particle Size:
3 µm
Pore Size:
100 Å
Temp.:
35 °C
Sample
phenoxyacid herbicides
Diluent:
acetonitrile
Conc.:
100 ppb
Inj. Vol.:
10 µL
Mobile Phase
A:
10mM ammonium acetate in water
B:
10mM ammonium acetate in methanol
Time (min.) %A %B
0
80 20
8
10 90
12
0 100
14.8
0 100
15
80 20
Flow:
0.5 mL/min.
Detector
Applied Biosystems/MDS Sciex LC/MS/MS
Model #:
4000 QTRAP™ LC/MS/MS System
Ion Source:
Electrospray
Ion Mode:
ESI-
Ion Spray Voltage: -4.2 kV
ESI Voltage:
-4.2 V
Gas 1:
50 psi (344.7 kPa)
Gas 2:
60 psi (413.7 kPa)
Source Temp.:
600 °C
Mode:
MRM
Instrument
Applied Biosystems/MDS Sciex LC/MS/MS System
Acknowledgement Work performed at Applied Biosystems
LC_EV0511
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Mar 2011
