710
HPLC CHROMATOGRAMS |
ENVIRONMENTAL
Aldehydes, Ketones
Carbonyls by CARB Method 1004 on Allure® AK
LC_EV0393
Ret. Time
Peak
(min.)
DNPH derivatives of:
1. formaldehyde
4.74
2. acetaldehyde
5.78
3. acrolein
6.86
4. acetone
7.09
5. propionaldehyde
7.31
6. crotonaldehyde
8.19
7. methacrolein
8.55
8. butyraldehyde
8.79
9. methylethylketone
9.06
10. benzaldehyde
10.03
11. valeraldehyde
10.39
12.
m
-tolualdehyde
11.08
13. hexaldehyde
11.36
Sample:
Inj.:
10µL
Conc.:
3µg/mL each analyte, as
aldehyde/ketone
Sample diluent: acetonitrile
Column:
Allure
®
AK
Cat.#:
9159525-700
Dimensions:
200mm x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
A) water : B) acetonitrile
Time (min.)
%B
0
60
8
70
10
100
Flow:
1.5mL/min.
Temp.:
30°C
Det.:
UV @ 360nm
Aldehydes and Ketones (DNPH derivatives) on Ultra C18
(40°C)
0
2
4
6
8
10
12
14 min.
LC_0036
1
2
4
5
7
8
3
6
9
10
11
12
13
Peak List:
DNPH derivatives of:
1. formaldehyde
2. acetaldehyde
3. acetone
4. acrolein
5. propionaldehyde
6. crotonaldehyde
7. MEK
8. methacrolein
9. butyraldehyde
10. benzaldehyde
11. valeraldehyde
12.
m
-tolualdehyde
13. hexanaldehyde
Sample:
Inj.:
10µL
Conc.:
3.0µg/mL each derivative
Column:
Ultra C18
Cat.#:
9174565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
Solvent A: water
Solvent B: acetonitrile
Solvent C: tetrahydrofuran
50-30% A/30-65% B/20-5% C, 0-15 min.
Flow rate:
1.5mL/min.
Det.:
UV @ 365nm
Temp.:
40°C
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
