Alcohol Metabolites on
Ultra II® Biphenyl (LC/MS)
Peak List
m/z
k'
Intensity
1. matrix
125
— —
2. ethyl glucuronide 221
2.75
220,408
3. ethyl sulfate
125
4.02
236,693
Sample:
Inj.:
5µL
Conc.:
5µg/mL each component
Sample diluent: urine diluted 1:10 with mobile phase
Column:
Ultra II
®
Biphenyl
Cat.#:
9609552
Dimensions:
50mm x 2.1mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Instrument:
Shimadzu Prominence UFLCXR
Mobile phase:
A: 5mM dihexylammonium acetate in water
B: 5mM dihexylammonium acetate in methanol
Time (min.)
%B
0.0
40
2.5
40
2.6
100
5.4
100
5.5
40
7.5
40
Flow:
0.3mL/min.
Temp.:
40°C
Det.:
Shimadzu LCMS-2010EV
Ion source:
ESI-
Mode:
SIM
Source temp.:
200°C
Source voltage: -4,000V
LC_CF0500
NEW!
Diuretics in Urine on Ultra II® Biphenyl
(LC/MS/MS, Positive Run)
Column
Ultra II
®
Biphenyl (cat.# 9609352)
Dimensions:
50 mm x 2.1 mm ID
Particle Size:
3 µm
Pore Size:
100 Å
Temp.:
40°C
Sample
Conc.:
500 ng/mL diuretics in urine, diluted 10x in
mobile phase
Inj. Vol.:
5 µL
Mobile Phase A: 0.1% formic acid in water
B: 0.1% formic acid in methanol
Time (min.) Flow (mL/min.) %A %B
0
0.5
90 10
6.00
0.5
0 100
6.1
0.5
90 10
8.00
0.5
90 10
Detector
Applied Biosystems/MDS Sciex LC/MS/MS
Model #:
API 5000
Ion Source:
TurboIonSpray
®
Curtain Gas:
25 psi (172.4 kPa)
Gas 1:
60 psi (413.7 kPa)
Gas 2:
40 psi (275.8 kPa)
Source Temp.:
550°C
Mode:
MRM
Dwell Time:
50 ms
Ion Mode:
ESI+
Ion Spray Voltage: 5.5 kV
LC_CF0509
MRM1 MRM2 MRM3
(Q1/Q3) (Q1/Q3) (Q1/Q3)
1. Amiloride
230/171 230/116 230/60
2. Triamterene 254/237 254/104 254/141
3. Indapamine 366/132 366/91 366/117
4. Ethacrynic acid 303/179 303/194 303/257
5. Bumetanide 365/240 365/156 365/284
Good retention for
hydrophilic compounds!
Diuretics on Ultra II® Biphenyl
(LC/MS/MS, Negative Run)
Ion Mode:
ESI-
Ion Spray Voltage: -4.5 kV
MRM1 MRM2 MRM3
(Q1/Q3) (Q1/Q3) (Q1/Q3)
1. Acetazolamide
221/83 221/80 221/58
2. Chlorothiazide
294/214 294/179 294/215
3. Hydrochlorothiazide 296/78 296/269 296/205
4. Bendroflumethiazide 420/289 420/328 420/197
5. Furosemide
329/285 329/205 329/78
LC_CF0510
NEW!
749
HPLC CHROMATOGRAMS |
CLINICAL/FORENSIC
Alcohol Metabol i tes, Diuret ics
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
