2
Performing Internal StandardCalibration using
PeakSimple version 3.51
Right clickanywhereon the chroma-
togram screenand select Components
Notice in theComponentswindow that
thenumber 2 is listed inparentheses
next to thepeaknumber 1.
This is howPeakSimple reminds you
that peak number 2 ( Butanetriol ) is the
Internal Standard for peak number 1
(Glycerin ).
It is agood ideaat this point to save the
Component table. ClickSaveandenter
a filename ( BioDiesel.cpt for example )
Return to theChromatogram screen,
point to theGlycerinpeakwith your
mouseand right click. Select Calibrate
Glycerin from themenu.