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Figure2
TheAllure
™
Basix column provides retention by a normal phasemechanism.
Figure3
TheUltraCyano column separates four of themost common calcium channel blockers.
LC_0091
LC_0062
Column:
Allure™ Basix
Catalog #:
9161565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
60Å
Conditions:
Mobile phase:
10mM ammonium
formate, pH 2.5:
acetonitrile
(20:80, v/v)
Flow:
1.2mL/min.
Temp.:
25°C
Det.:
UV @ 225nm
Peak List:
1. maleate salt
2. enalapril
Sample:
Inj.:
2.5µL
Conc.:
1.1mg/mL
Solvent:
methanol:water
(30:70)
Enalapril maleate, another common ACE inhibitor, can be
separated by polar interaction using the Allure
™
Basix phase.
The Allure
™
Basix column is able to interact with the basic
amide and amine of enalapril to provide retention by a
normal phase mechanism (Figure 2).
CalciumChannelBlockers
Verapamil, diltiazem, nicardipine, and nifedipine are a group
of calcium channel blockers used to treat high blood
pressure, angina (chest pain), and/or some arrhythmias. These
four compounds all contain a basic amine group. Addition-
ally, nifedipine and nicardipine contain more basic
nitrophenol and pyridine functional groups. Figure 3
demonstrates how basic functional groups can be used to
affect retention and separation of these compounds using the
Ultra Cyano column. Also, the Allure
™
Basix Column, in the
reverse phase mode, easily retains verapamil (Figure 4).
1
2
0
2
4 6
8 10 12 14min.
1
2
4
5
6
7
8
9
10 min.
3
4
5
Column:
Ultra Cyano
Catalog #:
9106565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
100Å
Conditions:
Mobile phase:
20mM potassium
phosphate monobasic,
pH 3.0:acetonitrile
(70:30, v/v)
Flow:
1.2mL/min.
Temp.:
30°C (± 1°C)
Det.:
235nm
Peak List:
1.
diltiazem
2.
nifedipine impurity
3.
verapamil
4.
nifedipine
5.
nicardipine
Sample:
Inj.:
5µL
Conc.:
100µg/mL
Solvent:
acetonitrile:water
(1:1)
