Table1
Typical retention times for common nucleotides, nucleosides, and purine and pyrimidine bases.
Compound
Retention Time
1
(min.)
CTP
1.5
UTP
1.5
CDP
1.6
UDP
1.6
GTP
1.7
TTP
1.8
CMP
1.9
GDP
1.9
UMP
2.0
TDP
2.2
ATP
2.3
GMP
2.6
Cytosine
2.6
ADP
2.7
Uracil
2.7
Cytidine
3.1
TMP
3.3
Uridine
3.6
AMP
4.2
Guanine
4.6
Thymine
4.6
Guanosine
7.4
Thymidine
9.0
Adenine
9.3
Adenosine
16.2
1. Retention times are for 150x4.6mm column; Flow rate: 1.0mL/min; Mobile phase:
20mM ammonium acetate, pH 5.8: Methanol. (97.5:2.5, v/v).
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