Peak List:
Conc. (µg/mL):
1. GTP
212
2. GDP
260
3. GMP
362
4. guanine
80
5. guanosine
226
Sample:
Inj.:
20µL
Solvent:
20mM ammonium
acetate, pH 5.8
RestekCorporation • (800)356-1688 • (814)353-1300 •
Figure3
Ultra IBD separates adenine, adenosine, ATP, ADP, and AMP with simple isocratic reverse phase conditions.
Figure4
Ultra IBD separates guanine, guanosine, GTP, GDP, and GMP with simple isocratic reverse phase conditions.
1
2
LC_0129
LC_0131
Adenosine is a ribonucleoside (adenine + ribose). ATP is a
particularly important nucleotide, serving as a universal
source of energy for biological processes.
The Ultra IBD column is particularly effective for retaining
and resolving complex mixtures of nucleotides, nucleosides,
and purine and pyrimidine bases. The unique Ultra IBD
stationary phase is composed of a polar group within, or
intrinsic to, an alkyl chain. The polar group gives extra
retention for many polar analytes as well as unique selectiv-
ity, a very high level of base deactivation, and compatibility
with highly aqueous mobile phases. The Ultra IBD column is
ideal for LC/MS because it often can resolve acidic, basic,
zwitterionic and/or neutral compounds in a single analysis
using simple mobile phases.
Figures 3 through 7 each show separations of one of the
major purines or pyrimidines from its respective ribonucleo-
Column:
Ultra IBD
Catalog #:
9175565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
100Å
Conditions:
Mobile Phase:
20mM ammonium
acetate, pH 5.8:
methanol (97.5:2.5)
Flow:
1.0mL/min
Temp.:
35°C
Det.:
UV @ 260nm
Peak List:
Conc. (µg/mL):
1. ATP
258
2. ADP
320
3. AMP
274
4. adenine
84
5. adenosine
254
Sample:
Inj.:
20µL
Solvent:
20mM ammonium
acetate, pH 5.8
1
2
3
4
5
0 2 4 6 8 10 12 14 16 18 min.
Column:
Ultra IBD
Catalog #:
9175565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
100Å
Conditions:
Mobile Phase:
20mM ammonium
acetate, pH 5.8:
methanol (97.5:2.5)
Flow:
1.0mL/min
Temp.:
35°C
Det.:
UV @ 260nm
0 2.5 5 7.5 10 min.
3
4
5
