0 2 4 6 8 10 12 14 16 18 min.
1
2
3
7
5
4
8
9
10
11
6
Figure8
Ultra IBD separates an 11-component mixture of nucleotides, nucleosides, and nitrogenous bases.
Figure7
Ultra IBD separates uracil, uridine, UTP, UDP, UMP with simple isocratic reverse phase conditions.
1
LC_0133
LC_0128
RestekCorporation • (800)356-1688 • (814)353-1300 •
components are volatile. Figure 8 shows that the Ultra IBD
column can resolve a mixture of 11 various nucleotides,
nucleosides, and bases using ultraviolet (UV) detection.
The unique stationary phase of the Ultra IBD column can
retain and resolve mixtures of nucleotides, nucleosides, and
purine and pyrimidine bases by RP/HPLC, using isocratic
Peak List:
Conc. (µg/mL):
1. UTP
360
2. UDP
360
3. UMP
284
4. uracil
90
5. uridine
230
Sample:
Inj.:
20µL
Solvent:
20mM ammonium
acetate, pH 5.8
Column:
Ultra IBD
Catalog #:
9175565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
100Å
Conditions:
Mobile Phase:
20mM ammonium
acetate, pH 5.8:
methanol (97.5:2.5)
Flow:
1.0mL/min
Temp.:
35°C
Det.:
UV @ 260nm
0 2 4 6 min.
2
3
4
5
Column:
Ultra IBD
Catalog #:
9175565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
100Å
Conditions:
Mobile Phase: 20mM ammonium
acetate, pH 5.8:
methanol (97.5:2.5)
Flow:
1.0mL/min
Temp.:
35°C
Det.:
UV @ 260nm
Peak List:
Conc. (µg/mL):
1. CDP
146
2. CMP
113
3. ATP
117
4. ADP
145
5. cytidine
155
6. TMP
157
7. AMP
125
8. guanine
36
9. guanosine
103
10. adenine
38
11. adenosine
115
Sample:
Inj.:
20µL
Solvent:
20mM ammonium
acetate, pH 5.8
elution with a simple, volatile mobile phase. A single set of
chromatographic conditions can resolve any of the common
purine or pyrimidine bases from its respective ribonucleoside
and mono-, di-, and triphosphate nucleotides.
