RestekCorporation • (800)356-1688 • (814)353-1300 •
less than 3 minutes on the Allure
™
Basix in reverse phase
mode (Figure 8). The retention of HCTZ would increase if
the organic modifier exceeded 50%, but solubility may
become a concern. Triamterene is less charged than HCTZ.
The amides present in the triamerene pteridine structures and
the amino groups make this molecule fairly polar. Under the
mobile phase conditions shown in Figure 8, triamterene is
easily resolved from the HCTZ.
Summary
The Allure
™
Basix, Ultra CN, and Ultra IBD columns can be
used to achieve an alternate and effective selectivity to alkyl
stationary phases for many types of cardiac medications.
Because all three of these phases display a characteristic
U-shaped profile for retention versus percentage of organic
solvent in mobile phase, the mobile phase content can be
modified to use either the normal or reverse phase mode if
solubility problems arise. The selectivity of the Allure
™
Basix and Ultra CN phases are based upon non-ionic polar
interactions from basic functional groups as well as hydro-
phobic interaction. Basic molecules—especially those
containing electron-deficient nitrogen complexes—can be
retained readily by a normal phase mechanism. The selectiv-
ity for the IBD phase can be adjusted for acids, bases,
zwitterionic, or neutral molecules.
Figure8
The Allure
™
Basix column easily resolves the diuretic admixture of triamterene and hydrochlorothiazide (HCTZ)
LC_0086
Peak List:
1. hydrochlorothiazide
2. triamterene
Sample:
Inj.:
1µL
Conc.:
500µg/mL
Solvent:
methanol:water:
tetrahydrofuran
(30:35:35)
Column:
Allure
™
Basix
Catalog #:
9161565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
60Å
Conditions:
Mobile Phase:
20mM ammonium
acetate, pH 4.5:
acetonitrile (65:35, v/v)
Flow:
1.2mL/min
Temp.:
25°C
Det.:
UV @ 225nm
1
2.5
3
3.5
4
4.5 min.
2
ApplicationNotes:
(#59511)
ImprovedHPLCAnalysis ofAnalgesics
(#59512)
TheUltra IBDColumnAllowsHPLC
SeparationofPolar andNon-PolarAnalytes from
theSameSample
(#59510)
HPLCStationaryPhaseSelection for the
Analysis of Steroids
(#59118)
Allure
™
PFPPropylHPLCColumnProvides
ImprovedLC/MSAnalyses ofBasicCompounds
Restek is your source for free technical literature
call 814-353-1300 or 800-356-1688, ext. 5
FastFacts:
(#59728)
HPLCMobilePhaseAccessories
(#59896)
Trident
™
IntegralHPLCGuardColumnSystem
(#59302)
HPLC andLC/MSColumnKits
(#59303)
Allure
™
AcidixHPLCColumns
(#59314)
Trident
™
DirectGuardColumnSystem
(#59614A)
Ultra IBDHPLCColumns
