environmental
Applications
note
RestekCorporation • (800) 356-1688 • (814)353-1300 •
#59101
environmental
GCAnalysisof USEPAMethod 619TriazineHerbicidesUsing the
Rtx
®
-CLPesticides andRtx
®
-CLPesticides2Columns
Triazineherbicides have risen inusagedue to their lower toxicity
and less persistance in the environment as compared to the
chlorophenoxyherbicides (USEnvironmentalProtectionAgency
Method8151). TheUSEPAMethod619 is a test commonly
performedbyenvironmental laboratories for theanalysisof
triazineherbicides inwastewater.Although thismethodwas
written for usewithpackedcolumns, capillarycolumnshaveall
but replaced them inmodern laboratoriesperforming thismethod.
1
2
3
4
5
7
Figure1
TheRtx
®
-CLPesticides andRtx
®
-CLPesticides2 columns, connected inparallel, resolveall
EPAMethod 619 target compounds in less than 24minuteswith a single injection.
6
30m, 0.32mm ID, 0.50µmRtx
®
-CLPesticides (cat.# 11139) and 30m, 0.32mm ID, 0.25µmRtx
®
-CLPesticides2 (cat.# 11324) with a 5m, 0.32mm ID guard
column (cat.# 10044) and a ‘Y’Press-Tight
®
connector (cat.# 20403). Direct injection using a Uniliner
®
sleeve (cat.#21303) and adaptor for anHP5890
(cat.# 20964).
On-column concentration:
50pg each compound.
Oven temp.:
100°C to 250°C@ 4°C/min. (hold 5min.);
Inj./det. temp.:
250°C/NPD@275°C;
Carrier gas:
hydrogen, 9.65psi constant pressure;
GC:
HP 6890 with purged packed injection port.
1
2
3
4
5
7
6
Rtx
®
-CLPesticides Column
Rtx
®
-CLPesticides2 Column
8
8
min. 18
19
20
21
22
23
Peak
Compound
CASNo.
1
prometon
1610-18-0
2
simazine
122-34-9
3
atraton
1610-17-9
4
propazine
139-40-2
5
atrazine
1912-24-9
6
terbuthylazine
5915-41-3
7
secbumeton
26259-45-0
8
simetryn
1014-70-6
9
ametryn
834-12-8
10
prometryn
7287-19-6
11
terbutryn
86-50-0
Table I
CompoundsDetermined byUSEPAMethod 619
Evenwhenusing capillary columns, separationof the 11 target
compounds listed inMethod619 canbedifficult due to their
similar structures.Most common capillary column stationary
phases donot have adequate selectivity to resolve these com-
pounds, and a confirmation columnof different selectivity canbe
difficult to find.
Restek specificallydesigned theRtx
®
-CLPesticides andRtx
®
-
CLPesticides2 columns for the analysis of theorganochlorine
pesticides.These stationaryphases arehighly selective for
compounds that containelectronegative substituents and,
correspondingly,workwell for the analysis of chlorophenoxy
herbicides, PCBs, and the analytes listed inUSEPAMethod619
(Table1).
Figure1 shows the analyses ofMethod619 target compounds on
theRtx
®
-CLPesticides andRtx
®
-CLPesticides2 columns,
connected inparallel in the sameGCovenusing a ‘Y’Press-
Tight
®
connector. This configuration is beneficial becauseboth
theprimary and confirmation analysis is performedunder the
same conditions, at the same time, using a single injectionport.
The combinationof theRtx
®
-CLPesticides andRtx
®
-
CLPesticides2 columns resolves all target compounds in less
than24minutes. These stationaryphases are thermally stable to
min. 18
19
20
21
22
23
9 10 11
9 10
11
