Now that the system
is calibrated, prepare
anactual BioDiesel
sample by placing
100ul of BioDiesel,
100ul of Tricaprin in
pyridine, 100ul of Bu-
tanetriol inpyridine,
and100ul ofMSTFA
in a vial. Allow 20
minutes for the reac-
tion thendilute to8ml
with heptane.
Inject 1ul togenerate
a chromatogram simi-
lar to theoneat the
right
Zoom in to the region
surrounding the
mono, di and triolien.
Stretch the total
mono, di and tri com-
ponent window so it
includes the small
peaks surrounding the
monoolien, diolien
and triolien. The
ASTM6584method
doesnot preciselyde-
finewhich peaks to
includeor exclude, so
there is some judge-
ment required on the
part of theoperator.
PerformingASTM 6584 free and total glycerin in
BioDiesel using anSRI GasChromatograph
andPeakSimple software
Themain biodiesel esters are the very large peaks
in this region of the chromatogram
The peaks surrounding themonoolien are
assumed to be other monoglycerides
