For Glycerin the Level 1 amount is 50, so enter
the number 50 in the top left cell of the calibra-
tion spreadsheet.
Then click theAccept New button on the right
of the screen. A number which is the ratio of
theGlycerin peak area divided by the area of
theButanetriol internal standard peakwill be
automatically calculated and entered in the
next cell to the right.
Go through the same procedurewith the
Monoolein, DiOlein, andTriOlein peaks.
TheMonoOlein calibration screen is shown at
right. Note that the Level 1 amount for
MonoOlein is 1000. For DiOlein andTriOlein-
the Level 1 amount is 500.
The table below taken fromTable 3 of ASTM
D6584-00 shows the calibration level amounts
for the 4 standard peaks.
Level Glycerin
Mono
Di
Tri
1
50
1000
500 500
2
150
2500
1000 1000
3
250
5000
2000 2000
4
350
7500
3500 3500
5
500
10000
5000 5000
Don’t forget to save the curves using a unique
file name for each one.
Once the calibration curve has been saved the
first time, it will be saved automatically thereaf-
ter when you hit theClose button.
PerformingASTM6584 freeand total glycerin in
BioDiesel usinganSRI GasChromatograph
andPeakSimple software version3.67
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