Figure 3—Lowbleed and fast analysis timesmake theRtx
®
-OPPesticides2 column an excellent choice for
GC/MS analysis of organophosphorus pesticides.
GC_EV00602
1. dichlorvos
2. hexamethylphosphoramide
3. mevinphos
4. trichlorfon
5. TEPP
6. demeton-O
7. thionazin
8. tributylphosphate (IS)
9. ethoprop
10. naled
11. sulfotepp
12. phorate
13. dicrotophos
14. demeton-S
15. monocrotophos
16. terbufos
17. dimethoate
18. dioxathion
19. fonophos
20. diazinon
21. disulfoton
22. phosphamidon isomer
(breakdown product)
23. dichlorofenthion
24. chlorpyrifosmethyl
25. phosphamidon
26. parathion-methyl
27. ronnel
28. fenitrothion
29. aspon
30. malathion
31. chlorpyrifos
32. trichloronate
33. parathion-ethyl
34. fenthion
35. merphos
36. chlorfenvinphos
37. crotoxyphos
38. stirofos
39. tokuthion
40. merphos oxone
(merphos breakdown product)
41. ethion
42. fensulfothion
43 bolstar
44. carbophenothion
45. famphur
46. triphenylphosphate (SS)
47. EPN
48. phosmet
49. leptophos
50. tri-
o
-cresyl phosphate
51. azinphos-methyl
52. azinphos-ethyl
53. coumaphos
Column:
Rtx
®
-OPPesticides2 30m, 0.25mm ID, 0.25µm (cat.# 11243)
Sample:
CustomMix, plus:
8140/8141OPPesticides CalibrationMixA (cat.# 32277)
8141OPPesticides CalibrationMixB (cat.# 32278)
Triphenylphosphate Standard (cat.# 32281)
Tributylphosphate Standard (cat.# 32280)
1µL, 100ppm each (100ng on column)
Inj.:
1.0µL splitless (hold 0.4min.), 4mm ID double
gooseneck inlet liner (cat.# 20785)
Inj. temp.:
250°C
Carrier gas:
helium, constant flow
Flow rate:
1.0mL/min.
Oven temp.:
80°C (hold 0.5min.) to 140°C@ 20°C/min.
to 210°C@ 4°C/min. (hold 1min.) to
280°C@ 30°C (hold 5min).
Det:
MS
Transfer line temp.:
280°C
Scan range:
35-400amu
Ionization:
EI
Figure 4—Adjusting the flow rate can affect elution time and resolution (and elutionorder) for
OPPs on anRtx
®
-OPPesticides2 column.
11.70
12.00
12.30
1 2
3
4,5 6
7
12.20 12.40 12.60 12.80 13.00
1
2
3
4,5 6
7
83cm/sec.
dead time 0.6min.
71cm/sec.
dead time 0.7min.
56cm/sec.
dead time 0.9min.
46cm/sec.
dead time 1.1min.
Column:
Rtx
®
-OPPesticides2 30m, 0.32mm,
32µm (cat.#11241)
Injector:
200°C split/splitless, purge off,
hold 1min
Oven temp.:
80°C (hold 0.5min.) to 280°C@
12°C/min. (hold 10min.)
Detector:
FPD@ 250°C
Sample:
0.5µL 200ng/mL of customOP
pesticidesmix described in
Figure 3 (GC_EV00602)
(partial chromatograms shown)
Other conditions as described for Figure 2
(GC_EV00394)
1. terbufos
2. dimethoate
3. diazinon
4. dioxathion
5. fonophos
6. disulfoton
7. phosphamidon isomer
10.40
10.80
11.10
1
2
3,4
5 6
7
11.80
11.60
11.40
11.20
11.00
1
2 3,4,5 6
7
Restek Corporation • (800) 356-1688 • (814) 353-1300 •
GC_EV00502A
GC_EV00502B
GC_EV00502C
GC_EV00502D
