ID df (µm)
temp. limits
15-Meter 30-Meter
0.25mm
0.25 -60 to 310/330°C 11540
11543
0.32mm
0.50 -60 to 310/330°C 11541
11544
0.53mm
0.50 -60 to 310/330°C 11542
11545
ID df (µm)
temp. limits
15-Meter 30-Meter
0.25mm
0.20 -60 to 310/330°C 11440
11443
0.32mm
0.25 -60 to 310/330°C 11441
11444
0.53mm
0.42 -60 to 310/330°C 11442
11445
ID
5-Meter
10-Meter
0.25mm
10026
10036
0.32mm
10027
10037
0.53mm
10028
10038
Qty.
Siltek
™
Siltek
™
Siltek
™
w/Siltek
™
Wool
w/Carbofrit
™
each
-214.1
-213.1
-216.1
5-pack
-214.5
-213.5
-216.5
25-pack
-214.25
-213.25
-216.25
Figure 2
For permission to reproduce any portion of this application note, please contact Restek’s publications/graphics department by phone (ext. 2128) or FAX.
RestekTrademarks:
MXT, Press-Tight, Siltek, Stx, and theRestek logo.
Stx
™
-CLPesticidesColumn
Stx
™
-CLPesticides2Column
30m, 0.32mm ID, 0.25µmStx-CLPesticides2 (cat# 11444)
Oven temp.:
120°C (hold 1min.) to 300°C@ 9°C/min.;
Detector:
310°C, Agilent ECD;
Inj.:
1µL, pesticide standardmix A and B (cat.#32003,32004, 20/40/200ng/mL);
Inj. temp.:
220°C (splitless, 1min. purge off hold);
Inlet liner:
4mmSiltek
™
single
gooseneck (cat.# 20798-214.1);
Dead time:
0.8min. @ 120°C.
1. 2,4,5,6-tetrachloro-m-xylene (ss)
2.
a
-BHC
3.
g
-BHC
4.
b
-BHC
5.
d
-BHC
6. heptachlor
7. aldrin
8. heptachlor epoxide
9.
g
-chlordane
10.
a
-chlordane
11. 4,4'-DDE
12. endosulfan I
13. dieldrin
14. endrin
15. 4,4'-DDD
Siltek
™
GuardTubing
Siltek
™
Inlet Liners
The key tomaximizing resolutionwith these columns is set-
ting the proper flow rate. The elution order of endosulfan I
and 4,4'-DDE on the Stx
™
-CLPesticides column are used to
indicate optimized flow rate; 4,4'-DDE should elute between
a
-chlordane and endosulfan I. If 4,4'-DDE and endosulfan I
are not adequately resolved, decreasing the column flowwill
improve separation. Once resolution is acceptable for these
two compounds, then the remaining compounds will be
resolved in a dual-column single injection port configuration.
Conclusion
Endrin breakdown and adsorption are caused by active sites
throughout the analytical system, especially the injection port
and column. Using Siltek
™
-treated liners, guard columns, and
Stx
™
-CLPesticides and Stx
™
-CLPesticides2 analytical columns
for chlorinated pesticides analyses results in a chromatographic
systemwith unsurpassed inertness, allowing for longer calibra-
tion periods.
For Siltek
™
-deactivation, include the suffix number shown above to the inlet
liner catalog number.
The Stx
™
-CLPesticides2 column is the ideal
confirmation column to the Stx
™
-CLPesticides column
for chlorinated pesticides analysis.
16. endosulfan II
17. 4,4'-DDT
18. endrin aldehyde
19. methoxychlor
20. endosulfan sulfate
21. endrin ketone
22. decachlorobiphenyl (ss)
Figure 3
Universal Press-tight
®
‘Y’connector andmetalMXT
®
‘Y’connector.
Description
Each 3-pack
Universal ‘Y’Press-tight
20405 20406
MXT low dead-volume ‘Y’ (0.28mm)
20396 —
MXT low dead-volume ‘Y’ (0.53mm)
20395 —
Connectors
7
8
5
6
3
4
1
2
15
16
13
14
11
12
9 10
21
22
19
20 17
18
min.
1
2
3
4
5
6
7
8
9
10
11
12
13
GC_EV00512
Stx
™
-CLPesticidesCapillaryColumnKits
Includes an Stx
™
-CLPcolumn, an Stx
™
-CLP2 column, Siltek
™
guard tubing, and aUniversal ‘Y’Press-Tight
®
Connector
0.25mm
(cat.# 11190);
0.32mm
(cat.# 11191);
0.53mm
(cat.# 11192)
