CabbageExtract
HPLC
UltraAqueousC18
FOODS, FLAVORS,
& FRAGRANCES
13
Vitamins
Phone: 800-356-1688 or 814-353-1300
Water-soluble vitamins include both acidic and
basic compounds, as well as some highly polar
molecules, e.g., ascorbic acid (vitaminC), thiamin
(B1), riboflavin (B2), nicotinic acid (B3), pyridox-
ine (B6), and folicacid.Theverypolar compounds
aredifficult to retainby reversedphaseHPLC, and
many methods call for ion-pairing reagents to
improve retention.
Ultra Aqueous C18
HPLC
columns resolve sixwater-soluble vitamins using a
gradient elutionprogram,without theneed for ion
pairing reagents.
Consumption of glucosinolates (
β
-thioglucoside
N-hydroxysulfate precursors of isothiocyanates) is
associated with a significantly reduced risk for a
varietyof cancers. Becauseglucosinolates arehigh-
lypolar, ionpairing reagentsare sometimesused to
retain themby reversedphaseHPLC.
Due to enhanced retention for polar compounds
and compatibility with 100% aqueous mobile
phases, an
UltraAqueousC18
column can sepa-
rate glucosinolates by reversed phase HPLCwith-
out ionpairing reagents.
pinnacle II
™
hplc
columns
Developed usingRestek silica.We strictly control the quality of rawmaterial,
phase bonding, and column packing. You’ll be impressedwith our column-to-
column reproducibility!
Formore information and applications,
request the Pinnacle II
™
Column flyer. (lit. cat.#59281)
Column:
Ultra Aqueous C18
Cat.#:
9178565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
A: 50mM potassium phosphate,
pH 2.5
B: acetonitrile
Time (min.):
%B
0.0
0
10
75
11
0
16
0
Flow:
1.0mL/min.
Temp.:
ambient
Det.:
UV@210nm
Peak List:
1. phenethyl glucosinolate
Sample:
Inj.:
20µL
Solvent:
water
LC_0165
restek
innovation
!
Monitor glucosinolateswithout ion
pairing reagents.
min.
for
more
info
Request ApplicationsNote AnalyzePolar Compounds by Reversed
PhaseHPLCUsingUltraAqueous C18Column (cat.# 59177).
