PhenolicAntioxidants
HPLC
Pinnacle II™C18
SorbicAcidandBenzoicAcid
HPLC
UltraPhenyl
min.
FOODS, FLAVORS, & FRAGRANCES
16
Phone: 800-356-1688 or 814-353-1300
Preservatives
Preservatives
Preservation techniques areused in foods andbev-
erages tomaintain thequalityof theproduct.Food
preservation can be done by both physical and
chemicalmeans.Physical techniquesmight involve
drying, heating, freezing, pasteurization, or irradi-
ation; chemical techniques include adding sugar,
salt, or preservatives. Several common chemicals,
such as acetic acid and citric acid, can be used to
prevent the growth of food-spoiling microorgan-
isms. Calcium propionate can be used to prevent
mold growth. In addition, benzoate and sorbate
salts can be used as mold inhibitors in a range of
food andbeverage products.
Benzoate and sorbate salts canbe analyzed in their
protonated form (i.e., as benzoic acid and sorbic
acid) by reversed phase HPLC using an
Ultra
Phenyl
column and acidified water:methanol
(80:20,v/v)as themobilephase.Bymonitoring the
UV absorbance at 245nm, sensitive detection of
benzoic and sorbic acids canbe achieved. Foropti-
mum sensitivity, monitor benzoic acid at 230nm
and sorbic acid at 254nm.
Analyze phenolic antioxidants by reversed phase
HPLC using a
Pinnacle II™ C18
column and an
acidicmobile phase.
LC_0150
Sample:
Inj.:
10µL
Conc.:
100 ppm sorbic acid,
200 ppm benzoic acid
Solvent:
mobile phase
Column:
Ultra Phenyl
Cat.#:
9105565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
1% acetic acid:methanol
(80:20, v/v)
Flow:
1.2mL/min.
Temp.:
ambient
Det.:
UV@245nm
Peak List:
1. sorbic acid
2. benzoic acid
min.
0
2
4
6
8
10
12
14
16
18
2
1
LC_0198
1
2
3
4
0
2
4
6
8
10
12
14
16
18min.
Peak List:
conc.: (ppm)
1. propyl gallate
168
2. TBHQ
182
3. 2-BHA+ 3-BHA 197
4. BHT
193
Sample:
Inj.:
10µL
Solvent:
methanol
Column:
Pinnacle
II
™
C18
Cat.#:
9214565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
110Å
Conditions:
Mobile phase: A= 1% acetic acid
B=methanol
Time B
(min.) (%)
0
50
4
50
10 90
25 90
26 50
Flow:
1.0mL/min
Temp.:
30°C
Det.:
UV@280nm
for
more
info
Request Flyer HighPerformance SilicaProducts (cat.# 59901).
