SugarAlcohols (TMSderivatives)
PACKEDGC
3%Rt-101on100/120Silcoport™
FOODS, FLAVORS,
& FRAGRANCES
11
Carbohydrates
Phone: 800-356-1688 or 814-353-1300
EitherGCorHPLC,dependingon theneedsof the
analyst and theequipment available, canbeused to
analyze carbohydrates.Typically, greater sensitivity
is possible using GC techniques, although sample
preparation will become more involved. HPLC
analysis using an amino-based stationary phase is
themost popular technique for the routine analy-
sis of simple sugars.This analysis involves isocratic
elution (e.g.,acetonitrile:water,75:25)anda refrac-
tive index detector (RID). An
Ultra Amino
or
Pinnacle II™Amino
column canbe used to sepa-
rate fructose,glucose, sucrose,maltose,and lactose.
Good resolution can be achieved in less than 15
minutes using a flow rate of 0.8mL/min. This
method is applicable to a wide range of food and
beveragematrices.
Because sensitivity can be limited when using a
refractive index detector, GC often is used for
trace-level analyses of simple sugars and sugar
alcohols after derivatization, which makes the
compounds more volatile and thermally stable.
Alditol acetatederivativesof sugarscanbeanalyzed
using an
Rtx®-225
capillary column, a
cyanopropyl-containing siloxane phase. The TMS
derivatives of simple sugars, complex sugars, and
sugar alcohols can be analyzed using a bonded
packed column, such as
3%Rt-101 on 100/120
Silcoport™
packing.
5
GC_FF00128
87
6
4
3
2
1
Peak List:
1. rhamnitol
2. fucitol
3. ribitol
4. arabinitol
5. mannitol
6. galactitol
7. glucitol
8. inositol
15m, 0.25mm ID, 0.25µmRtx
®
-225 (cat.# 14020)
0.5µL split injection
Oven temp.:
190°C (hold 5min.) to 250°C
@8°C/min. (hold 5min.)
Inj. & det. temp.:
260°C
Carrier gas:
hydrogen
Linear velocity:
42cm/sec. set@40°C
FID sensitivity:
16 x 10
-11
AFS
Split ratio:
50:1
min.
2.5
5
7.5
10
12.5
1
2
3
4
5
6
7
8
3%Rt-101 on 100/120 Silcoport
™
(cat.# 80400)
2m,
1
/
8
" OD x 2mm ID
Oven temp.:
140°C to 230°C@5°C/min.
(hold 5min.)
Inj./det. temp.:
250°C / 300°C
Det.:
FID
Carrier gas:
helium
Column flow rate: 35mL/min.
FID sensitivity:
1.28 x 10
-10
AFS
Peak List:
1. l-arabinose
2. xylitol
3. d-arabinitol
4. d-mannose
5. l-sorbose
6. d-glucose
7. d-sorbitol
8. inositol
GC_FF00130
min.
5
10
15
20
Sugars (asalditol acetates)
GC
Rtx®-225
