FAMEs (CocoaButter)
GC
Stabilwax®
FAMEs (MarineOil)
GC
FAMEWAX™
FOODS, FLAVORS, & FRAGRANCES
4
Phone: 800-356-1688 or 814-353-1300
Fats & Oils
Fattyacidmethylesters (FAMEs)
FAMEs analysis is important in fats andoils charac-
terization and in the determinationof total fat con-
tent in foods. To prepare themethyl esters, fats are
extracted frommatrices using non-polar solvents,
and saponified to produce free fatty acids. After
derivatization to the methyl esters, molecules have
increased volatility and decreased activity, which
permitsmore accuratequantitationbyGC.
Capillary columns withpolyethylene glycol (PEG)
or Carbowax® stationary phases are used to ana-
lyze saturatedandunsaturatedFAMEs.For the res-
olution of the
cis
and
trans
isomers, bis-
cyanopropyl phases typicallyareused.
Stabilwax®
and
Rtx®-Wax
columns provide excellent resolu-
tionof FAMEsderived frombothplant andanimal
sources.
FAMEWAX®
columns offer excellent reso-
lutionof polyunsaturatedFAMEswith significant-
ly reduced analyses times, compared to traditional
Carbowax® stationary phases. Individual
cis
and
trans
isomers are resolvedon an
Rt-2560
column,
making it the column of choice for analyzing par-
tiallyhydrogenated fats.
GC_FF00568
Peak List:
1. C14:0
2. C15:0
3. C16:0
4. C16:1
5. C16:2
6. C17:0
7. C17:1
8. C16:4
9. C18:0
10. C18:1 (oleate)
11. C18:1 (vaccenate)
12. C18:2n6
cis
13. C18:3n3
14. C18:4n6
15.C18:4n3
16. C20:0
17. C20:1n7
18. C20:1n9
19. C20:4n6
20. C20:4n3
21. C20:5n3
22. C22:1n7
23. C21:5n3
24. C23:0 (IS)
25. C22:5n6
26. C22:5n3
27. C22:6n3
28. C24:1
FAMEWAX
™
30m, 0.32mm ID, 0.25µm (cat.# 12498)
Sample:
12mg/mL total FAMEs
Inj.:
0.5µL, split (150:1), 3mm ID split liner forTrace Series GCs,
packedwith glasswool (cat.# 20936-202.1)
Inj. temp.:
250°C
Carrier gas:
hydrogen, constant flow
Linear velocity:
62cm/sec.
Oven temp.:
195°C to 240°C@ 5°C/min. (hold 1min.)
Det.:
FID, 250°C
restek
innovation
!
FAST analysis ofmarine oil FAMEs!
30m, 0.25mm ID, 0.25µm Stabilwax
®
(cat.# 10623)
1.0µL split injection of a FAME reference
standard for cocoa butter.
Oven temp.:
200°C to 250°C@8°C/min.
(hold 3min.)
Inj. & det. temp.: 250°C
Carrier gas:
hydrogen
Linear velocity: 31.4cm/sec. set@200°C
FID sensitivity: 8 x 10
-11
AFS
Split ratio:
45:1
Peak List:
1. C14:0
2. C16:0
3. C16:1n7
4. C17:0
5. C18:0
6. C18:1n9
7. C18:2n6
8. C18:3n3
9. C20:0
10. C20:1n9
11. C22:0
GC_FF00120
1
3
2
6
7
5
9
10
11
4
min.
2
4
6
8
8
ordering
note
To order one of the columns highlighted on pages 1 through 7,
please see the following pages:
FAMEWAX
™
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
Rtx
®
-65TG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32
Rtx
®
-200 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
Rtx
®
-2560 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
Rtx
®
-Wax . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
Stabilwax
®
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
Stabilwax
®
-DA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
min.
for
more
info
Request ApplicationsNote Analyzing Fatty AcidMethyl Esters
(cat.# 59584A).
