Triglycerides
GC
Rtx®-65TG
FOODS, FLAVORS,
& FRAGRANCES
3
Fats & Oils
Phone: 800-356-1688 or 814-353-1300
Triglycerides
Triglycerides are naturally-occurring esters of fatty acids and glycerol, and themain component (90-95%) of dietary lipids.Mono-
and diglycerides also are esters, but contain one or two fatty acid groups, respectively. Triglycerides are classified according to the
nature of their esterified fatty acids. The fatty acid groups in the triglyceridemolecule can be classified as saturated or fully hydro-
genated (e.g.,C14:0),orunsaturated (e.g.,C18:1orC18:2).
In general, capillaryGC columns are the preferred tool for triglyceride analyses, providing shorter analyses times, higher efficiency,
andbetter quantitation thanpacked columnGC,HPLC, or supercritical fluid chromatography (SFC). Sample preparation ismini-
mal, involving liquefying the samplebeforedilutingwith a solvent such as dichloromethaneor isooctane.Additional sampleprepa-
ration is necessary ifmono- anddiglycerides and free fatty acids arepresent in significant amounts.Because these compounds have
relativelyhighmolecularweights andpolarities that increasewith thedegreeof unsaturation,highoven temperatures arenecessary.
An
Rtx®-65TG
(65%phenyl/35%methyl polysiloxane) column is able to resolve triglycerides according todegree of unsaturation,
as well as according to carbon number. The extended thermal stability of this column allows the use of a high oven temperature,
yielding short analyses times. In addition, the advanceddeactivation techniquesused toprepare
Rtx®-65TG
columns result in lower
bleed and longer column lifetimes than for traditional columns.
Formore information, requestApplicationsNote
GCAnalysisofTriglycerides
(cat.#59580A).
for
more
info
Restek offers an extensive line of GC andHPLC columns, accessories, and replacement parts. Call to
request one of these catalogs for a full listing of products or visit us on theweb at
Peak List:
1. POP
2. PLP
3. POO
4. PLO
5. PLL
6. OOO
7. OLO
8. OOL
9. LLL
a) canolaoil
b) cornoil
c)oliveoil
min.
4
8
4
5
6
7
8
9
1
2 3
6
4
5
8
7
7
9
1
2
3
4
6
7
8
GC_FF00136
15m, 0.25mm ID, 0.10µmRtx
®
-65TG (cat.# 17005)
0.8µL split injection of triglycerides.
Oven temp.:
a) 355°C (hold 1min.) to 365°C@1°C/min.
b) 350°C (hold 0.5min.) to 365°C@1°C/min.
c) 350°C (hold 1min.) to 365°C@0.5°C/min.
Inj. & det. temp.:
365°C
Carrier gas:
hydrogen
Linear velocity:
50cm/sec.
FID sensitivity:
8 x 10
-11
AFS
Split ratio:
40:1
min.
4
8
min.
4
8
HPLCProducts
Lit. Cat. #59241B
2005General Catalog
Lit. Cat. #59065
AgilentGCReplacement
Parts, Lit. Cat. #59627E
