Sterols (Cholesterol)
GC
Rtx®-225
FOODS, FLAVORS,
& FRAGRANCES
9
Fats & Oils
Phone: 800-356-1688 or 814-353-1300
15m, 0.25mm ID, 0.25µmRtx
®
-225 (cat.# 14020)
1.5µL split injection of neutral sterols and phytosterols,
200ng on-column
Oven temp.:
260°C
Inj. & det. temp.:
260°C
Carrier gas:
helium
Linear velocity:
45cm/sec. set@240°C
FID sensitivity:
8 x 10
-11
AFS
Split ratio:
30:1
min.
0
10
Peak List:
1. 5-
α
-cholestane
2. coprosterol
3. cholesterol
4. brassicasterol
5. coprostanone
6. campesterol
7. stigmasterol
8.
β
-sitosterol
2
3 4
5
7
8
1
6
GC_FF00431
Peak List:
1. cholesterol
2. brassicasterol
3. campesterol
4. stigmasterol
5.
β
-sitosterol
60m, 0.25mm ID, 0.25µmRtx
®
-5 (cat.# 10226).
1µL splitless injection*
Oven temp.:
200°C (hold 1min.) to 340°C@15°C/min. (hold 10min.)
Inj./FID temp.: 345°C/355°C.
*Split injectionmay be used, but results can have greater variability. A split
flow of 112mL/min. is suggested.
1
2
3
4
5
min.
5
10
GC_FF00368
Chromatogram provided by the Institute for Nutraceutical Advancement (INA)
Phytosterols (SawPalmetto)
GC
Rtx®-5
In addition to themethod for fatty acid content in
saw palmetto (page 6), the INA has published a
method for thedeterminationof sterols in sawpal-
mettobyGC.This assay canbe applied to stigmas-
terol, campesterol, brassicasterol, and
β
-sitosterol
in sawpalmetto fruit,oil extract, andblendedpow-
ders. The sample is analyzed after hydrolysis,
saponification, and derivatization of the sterols.
For this assay, an
Rtx®-5
column is used. This col-
umn features the thermal stability needed to pro-
vide accurate quantitation of the phytosterols
(340°C).
for
more
info
Request ApplicationsNote The Institute for Nutraceutical
Advancement (INA) ValidatesGCMethods for SawPalmetto
UsingRtx
®
-5 and Stabilwax Columns (cat.# 59136).
