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GC COLUMNS | APPLICATION-SPECIFIC COLUMNS
Foods, Flavors, & Fragrances
Rtx®-65TG Columns (fused silica)
(high-polarity phase; Crossbond® diphenyl dimethyl polysiloxane)
• Application-specific columns, specially tested for triglycerides.
• Stable to 370 °C.
The Rtx®-65TG phase resolves triglycerides by degree of unsaturation as well as by car-
bon number. Because of the chemistry required to achieve 370 °C thermal stability, an
Rtx®-65TG column should not be used for the analyses of polar compounds.
Rtx®-65TG Structure
CH3
CH3
Si
35%
O Si
65%
O
Triglycerides in Foods Analysis
crossbond® technology
Reduces bleed, prolongs column lifetime,
and allows rejuvenation through solvent
rinsing.
please note
Triglycerides are often injected via on-column injection. Use 0.53 mm retention gaps
and appropriate connectors.
• Vu2 Union® connectors (see )
• MXT®-Union connector kits for fused silica (see page )
Similar to: (65%-phenyl)-methylpolysiloxane
Egg Pasta Sterols & Glycerides on Rtx®-65TG
GC_FF01046
Column Rtx®-65TG, 30 m, 0.25 mm ID, 0.10 µm (cat.# 17008)
Sample Fat extract from egg pasta in diethyl ether solution with 3,000 ppm squalene (IS)
Conc.: 50 µg/mL
Injection
Inj. Vol.: 0.5 µL ptv split (split ratio 80:1)
Inlet Temp.
Program: 70 °C (hold 12 min) to 370 °C at 99 °C/min (hold 5 min)
Oven
Oven Temp.: 220 °C (hold 2 min) to 360 °C at 5 °C/min (hold 5 min)
Carrier Gas H2, constant flow
Flow Rate: 1.5 mL/min
Detector FID@ 370 °C
Acknowledgement
Daniele Naviglio, Fabiana Pizzolongo; Dipartimento di Scienza degli Alimenti –
Università degli Studi di Napoli “Federico II” – Via Università, 100 - 80055 Portici
(NA) – Italia
Peaks
1. Squalene (IS)
2. Cholesterol
3. β-Sitosterol
4. Diglycerides
5. Triglycerides
15-Meter 30-Meter
ID df temp. limits cat.# price cat.# price
0.25 mm 0.10 µm 40 to 370 °C 17005 17008
0.32 mm 0.10 µm 40 to 370 °C 17006 17009
0.53 mm 0.10 µm 40 to 370 °C 17007 17010
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