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www.restek.comGC COLUMNS |
APPLICATION-SPECIFIC COLUMNS
Foods, Flavors, & Fragrances
Rxi®-624Sil MS Columns
(fused silica)
(midpolarity Crossbond® phase)
• Low-bleed, high-thermal stability column—maximum temperatures up to 320 °C.
• Inert—excellent peak shape for a wide range of compounds.
• Selective—G43 phase highly selective for volatile organics and residual solvents,
great choice for USP<467>.
• Manufactured for column-to-column reproducibility—well-suited for validated
methods.
Rxi®-624Sil MS (G43) Structure
x
Si
O
(CH
2
)
3
CH
3
CH
3
CH
3
Si
O
R
O
CH
3
CH
3
Si
94%
y
CH
3
CH
3
Si
C N
similar phases
DB-624, VF-624ms, CP-Select 624 CB
20-Meter
30-Meter
60-Meter
75-Meter
105-Meter
ID df
temp. limits cat.#
price
cat.#
price
cat.#
price
cat.#
price
cat.#
price
0.18 mm 1.00 µm -20 to 300/320 °C 13865
0.25 mm 1.40 µm -20 to 300/320 °C
13868
13869
0.32 mm 1.80 µm -20 to 300/320 °C
13870
13872
0.53 mm 3.00 µm -20 to 280/300 °C
13871
13873
13874
13875
Terpenes Analysis for Cannabis and Hops
Similar to: (6%-cyanopropylphenyl)-methylpolysiloxane
1
2
3
4 5
6
7
8
9
10
11
12
13 14
8
8.2
8.4 8.6
8.8
9
15
16 17
10.6 10.8 11 11.2 11.4 11.6 11.8 12 12.2
18
19 20 21
22 23
24,25 26
27
28
29 30
31
32
33
34
35
36
8
9
10
11
12
13
14
15
16
Time (min)
Medical Cannabis Terpenes on Rxi®-624Sil MS by FET-HS-GC
GC_FS0518
Column
Rxi® -624Sil MS, 30 m, 0.25 mm ID, 1.40 µm (cat.# 13868)
Sample
Terpenes mix
Diluent:
Isopropyl alcohol
Conc.:
200 ng/µL (0.02% wt/vol). The sample was prepared by placing
10 µL into the headspace vial.
Injection
headspace-loop split (split ratio 10:1)
Liner:
Sky® 1.0 mm ID straight inlet liner (cat.# 23333.1)
Headspace-Loop
Inj. Port Temp.:
250 °C
Instrument:
Tekmar HT-3
Inj. Time:
1.0 min
Transfer Line Temp.: 160 °C
Valve Oven Temp.: 160 °C
Needle Temp.:
140 °C
Sample Temp.:
140 °C
Sample Equil. Time: 30.0 min
Vial Pressure:
20 psi
Loop Pressure:
15 psi
Oven
Oven Temp.:
60 °C (hold 0.10 min) to 300 °C at 12.50 °C/min (hold 3.0 min)
Carrier Gas
He, constant flow
Linear Velocity:
33 cm/sec
Detector
FID@ 320 °C
Make-up Gas
Flow Rate:
45 mL/min
Make-up Gas Type: N
2
Hydrogen flow:
40 mL/min
Air flow:
450 mL/min
Data Rate:
20 Hz
Instrument
Agilent/HP6890 GC
Peaks
t
R
(min)
1.
α
-Pinene
7.39
2. Camphene
7.71
3.
β
-Myrcene
7.98
4. Sabinene
8.02
5.
β
-Pinene
8.11
6.
α
-Phellandrene
8.4
7.
δ
3-Carene
8.44
8.
α
-Terpinene
8.57
9. Ocimene
8.61
10. Limonene
8.71
11.
p
-Cymene
8.75
12.
β
-Ocimene
8.82
13. Eucalyptol
8.91
14.
γ
-Terpinene
9.06
15. Terpinolene
9.47
16. Linalool
9.87
17. Fenchone
10.06
18. Isopulegol
10.73
19. dl-Menthol
11.08
20. Borneol
11.19
21.
α
-Terpineol
11.29
22. Dihydrocarveol
11.40
23. Citronellol
11.51
24. Geraniol
11.82
25. 2-Piperidinone
11.88
26. Citral 1
11.92
27. Pulegone
11.97
28. Citral 2
12.24
29. Citral 3
13.19
30. Citral 4
13.43
31.
β
-caryophyllene
13.83
32.
α
-Humulene
14.21
33. Nerolidol 1
14.78
34. Nerolidol 2
15.08
35. Caryophyllene oxide 15.92
36.
α
-Bisabolol
16.43