AdvancedGasChromatography–Progress inAgricultural, Biomedical and Industrial Applications
48
The separation of the methyl esters of the fatty acids of pig testis lipids on the Carbowax
20M™ column is illustrated in Figure 3. A gas chromatograph Carlo Erba Model 4130
split/splitless injection system was used. The carrier gas was hydrogen (1 ml/min),
programme temperaturewas 165ºC for 3min and then temperaturewas raised at a rate of
4ºC/min to 195ºC andmaintained for 23min.
The three 16:1 isomers are well separated and distinct from the C
17
fatty acids. The C
18
components are separated from a minor 19:1 fatty acid and C
20
unsaturated constituents.
The 20:3(n-3), which co-chromatographswith 20:4(n-6) are just separable on a slightlymore
polar Silar 5CP™ column. C
22,
important fatty acids are cleanly separated (Christie, 1989).
Peaks canbe identified inTable 10 (Christie, 1989).
Equivalent chain-lengthsof themethyl ester derivatives of some fatty acids
No
Fatty acid
Silicone
Carbowax
Silar 5CP
CP-Sil 84
1.
14.00
14.00
14.00
14.00
14.00
2
14-isobr
14.64
14.52
14.52
14.51
3
14-anteiso
14.71
14.68
14.68
14.70
4
14:1(n-5)
13.88
14.37
14.49
14.72
5
15:0
15.00
15.00
15.00
15.0
6
16:0
16.00
16.00
16.00
16.00
7
16-isobr
16.65
16.51
16.51
16.50
8
16-anteiso
16.73
16.68
16.68
16.69
9
16:1(n-9)
15.76
16.18
16.30
16.48
10
16:1(n-7)
15.83
16.25
16.38
16.60
11
16:1(n-5)
15.92
16.37
16.48
16.70
12
16:2(n-4)
15.83
16.78
16.98
17.47
13
16:3(n-3)
15.69
17.09
17.31
18.06
14
16:4(n-3)
15.64
17.62
17.77
18.82
15
17:0
17.00
17.00
17.00
17.00
16
17:1(n-9)
16.76
17.20
17.33
17.50
17
17:1(n-8)
16.75
17.19
17.33
17.51
18
18:0
18.00
18.00
18.00
18.00
19
18:1(n-11)
17.72
18.14
18.24
18.40
20
18:1(n-9)
17.73
18.16
18.30
18.47
21
18:1(n-7)
17.78
18.23
18.36
18.54
22
18:2(n-6)
17.65
18.58
18.80
19.20
23
18:2(n-4)
17.81
18.79
18.98
19.41
24
18:3(n-6)
17.49
18.85
19.30
19.72
25
18:3(n-3)
17.72
19.18
19.41
20.07
26
18:4(n-3)
17.55
19.45
19.68
20.59
27
19:1(n-8)
18.74
19.18
19.32
19.47
28
20:1(n-11)
19.67
20.08
20.22
20.35
29
20:1(n-9)
19.71
20.14
20.27
20.41
30
20:1(n-7)
19.77
20.22
20.36
20.50
