6
Figure 12: PCMX inhand soaponPinnacle
™
DBC18
Restek Corporation • (800) 356-1688 •
ThePinnacle
™
DB line currently is availablewith threebondedphas-
es:C18,C8, and cyano. Figures 12–14 are representative“real world”
chromatograms for eachof thesephases.
Large, 140Å, poresmakePinnacle
™
DBmaterial an excellent choice
when you are seeking to transfer an analysis to a shorter column, for
faster separations. Figure 15 shows vanilla flavor profiles canbe
obtained in less than6minutes on a 50x4.0mm column.These
analyses typically areperformedon a 150x4.6mm column, and
analysis times approach20minutes.
SatisfactionGuaranteed
Chromatographic andphysical data comparisons showPinnacle
™
DB
andHypersil
®
BDSmaterials are very closelymatched, butwewant
you tobe completely comfortable in evaluatingour new columns.
We guarantee separations of your samples onPinnacle
™
DB columns
will be comparable to separationswithHypersil
®
BDS columns. If a
Pinnacle
™
DB columndoes notmeet your satisfaction, simply supply
uswith copies of chromatograms for your applicationon your
Pinnacle
™
DB column andon yourHypersil
®
BDS column.Wewill
credit your account for thepriceof thePinnacle
™
DB column—and
you cankeep the column, free!
If youare looking for a second source forHypersil
®
BDS-type columns,
or if you simplyare looking for a rugged,highquality,base-deactivated
material froma supplierwho canprovide columnsquickly,Pinnacle
™
DB columns areyour answer.Of course, as always, you'll getPlus 1
™
service andprompt, expert technical helpwhenyoudealwithRestek.
Figure 11: Xanthenes onPinnacle
™
DB andHypersil
®
BDSC18 columns.
1
2
3
4
8
6
4
2
0
min.
Column:
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
140Å/130Å
Conditions:
Mobile Phase:
20mMKH
2
PO
4
, pH 3:methanol (20:80 v/v)
Flow:
1.0mL/min
Temp.:
ambient
Det.:
UV@ 254nm
Sample:
Inj.:
2µL
SampleDiluent:
water:methanol (75:25 v/v)
SampleTemp.:
ambient
LC_0288
0
2
4
6
1
2
3
4
8 min.
LC_0287
RetentionTimes
PeakList:
Conc.
PinnacleDB Hypersil BDS
1. theobromine
200µg/mL
3.141
3.278
2. theophylline
390µg/mL
5.117
5.336
3.
β
-hydroxytheophylline 390µg/mL
5.605
5.860
4. caffeine
390µg/mL
7.936
8.298
Pinnacle
™
DBC18Column
(cat.# 9414565)
Hypersil
®
BDSC18Column
(cat.# 25105-154630*)
0
2
1
4
6
8 min.
PeakList:
1. PCMX (4-chloro-3,5-dimethylphenol)
Column:
Cat. #:
9414565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
140Å
Conditions:
Mobile Phase:
water:methanol (35:65 v/v)
Flow:
1.0mL/min.
Temp.:
ambient
Det.:
UV@ 280nm
Sample:
Inj.:
10µL
Conc.:
5% solution of hand soap inmethanol
SampleDiluent: methanol
SampleTemp.:
ambient
LC_0293
Nearly identical retention,
peak symmetry, and
efficiency!
Cleaning IndustryApplication
