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References
1. D. S. Christmore, R. C. Kelly, and L.A. Doshier, Journal of Forensic
Science, Volume 29, No. 4, October 1984, pp.1038-1044.
Product Listing
Rtx
®
-BAC1GCColumns
Length
ID
df (µm)
cat.#
30m
0.53mm
3.0
18001
30m
0.32mm
1.8
18003
Rtx
®
-BAC2GCColumns
Length
ID
df (µm)
cat.#
30m
0.53mm
2.0
18000
30m
0.32mm
1.2
18002
Hydroguard
™
FusedSilicaTransferLines
Length
ID
OD
cat.#
5m
0.18mm
0.34mm 10078
5m
0.25mm
0.37mm 10079
5m
0.32mm
0.45mm 10080
5m
0.53mm
0.69mm 10081
SplitlessSleeves forPerkin-ElmerGCs
Description
each
5-pk.
25-pk.
2mm ID
20730
20731 20732
Auto SYS 2mm IDw/wool
20829
20830 20831
AutoSYSXL 2mm ID 21717
21718 ——
Dual-ColumnAnalysisReplacement Ferrules
Two-hole
Fits column Graphite
Vespel
®
/
ferrule size
ID
Graphite
0.4mm (
1
/
16
")
0.25mm 20235 (5-pk.) 20241 (5-pk.)
0.5mm (
1
/
16
")
0.32mm 20235 (5-pk.) 20242 (5-pk.)
0.8mm (
1
/
8
")
0.53mm 20245 (5-pk.) 20246 (5-pk.)
HeadspaceAutosamplerVials&Seals
Description
100-pk. 1000-pk.
10mL clear vial, rounded bottom
21164 21165
silver seal w/PTFE/Silicone septa
21763 21764
Simulatedblood alcohol sampleswereprepared and analyzed
using amodificationof aprocedurepublishedbyChristmore et
al.
1
n
-Propanolwas used as the internal standardandwas
preparedat aconcentrationof 0.03g/dL in1.0Mammonium
sulfateas adiluent. Fivemillilitersof diluentwas added to1mLof
sample ina20mLheadspacevial.
PerformanceResults
ThePEAutoSYSGC andHS40headspace autosampler,
combinedwithRestek’sRtx
®
-BAC1andRtx
®
-BAC2columns,
provided excellent accuracy andprecision in the analysis of
blood alcohol.Calibration curveswere constructedusing
concentrations ranging from0.01% to0.5% ethanol.Correlation
coefficients above0.999were easilyobtained for all compounds.
Response factor repeatabilitywas less than +1% standard
deviationwhile analyzing six samples at a concentrationof 0.2%
Restek Trademarks:
Hydroguard, Rtx.
Other Trademarks:
Vespel, E.I. du Pont deNemours&Co., Inc.
ethanol. Basedonour experimentation, a systemdetection limit
of 0.001% ethanol shouldbe achievablewhilemaintaining a
minimum signal-to-noise ratioof 10.
Conclusion
Successful dual-columnconfirmational bloodalcohol analysis
canbe achieved in less than3minutes by combiningRestek’s
0.32mm IDRtx
®
-BAC1andRtx
®
-BAC2columnswith the
Perkin-ElmerAutoSYS andHS40headspace autosampler.
