1
HPLC
Applications
note
Restek Corporation • (800) 356-1688 •
#59742
Pinnacle
™
DBHPLCColumns asReplacements for Hypersil
®
BDS
Why choose Pinnacle
™
DB columns?
• Performance—equivalent replacement forHypersil
®
BDS columns.
• Superior base deactivation and low totalmetals content-
ideal for basic compounds.
• Excellent lot-to-lot and column-to-column reproducibility.
• In stock, ready to ship - 90%of columns ship same
day/next day of order.
• Wide selectionof stock columndimensions, including
semi-prep andprep scale.
• ISO 9001:2000 registered facility.
• Satisfaction guaranteed.
Whenwe set out to create a newbase-deactivated silica,we had five
specific goals:
• Amaterial that wouldmatchor exceed the performance
of any base-deactivated“Type B”silica.
• Amaterial that wouldprovide chromatographic separations
equivalent toHypersil
®
BDSmaterial.
• Amaterial with lowmetals content, for sharp, symmetric
peaks for basic analytes.
• A ruggedmaterial compatiblewith the pressures and
liquid environment of typicalHPLC applications.
• An effective and efficientmanufacturing process that gives
consistent, reproducible quality.
We have attained eachof these goals.
Pinnacle
™
DB silicawillmeet or exceed theperformanceof anyType
Bbase-deactivated silica in its class. InFigure 1, peak symmetry for
thebasic compoundpyridine is significantlybetter, and analysis time
is notably shorter on thePinnacle
™
DBC18 column thanon a typical
TypeBC18 column. Expect similar results in your own comparisons.
We havematched the desirable physical characteristics of Hypersil
®
BDSmaterial. Figures 2 and 3 show the excellent agreement
betweenparticle size andpore size distributions for Pinnacle
™
DB
andHypersil
®
BDSmaterials.
Figure 1: Pyridine/phenol testmix.
Figure 2: Equivalent particle size distribution for Pinnacle
™
DB silica andHypersil
®
BDS silica.
Particle Size analysis
PinnacleDB Hypersil BDS
GeometricMean (µm):
4.81±1.20
4.89±1.70
ArithmeticMean (µm):
4.89±0.94
4.95±0.82
Median (µm):
4.69
4.81
Mode:
4.69
4.79
MaxDiff. (%):
6.04
7.11
PinnacleDB
Hypersil BDS
LC_0245
PeakList:
Conc.
1. uracil
5.0µg/mL
2. pyridine
0.1µL/mL
3. phenol
1.86mg/mL
Sample:
Inj.:
5.0µL
SampleDiluent: mobile phase
Column:
TypeBC18
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
100Å
Conditions:
Mobile Phase:
A: 20mM potassium phosphate, pH 7.0
B: acetonitrile
Isocratic: 80%A:20%B
Flow:
1.0mL/min
Temp.:
ambient
Det.:
UV@ 254nm
LC_0248
Column:
Pinnacle
™
DBC18
Catalog #:
9414565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
140Å
Figure 3: Pinnacle
™
DB silica andHypersil
®
BDS silica have
remarkably equivalent pore volume distribution.
Pore Size analysis: Pinnacle
™
DB
SurfaceArea (m2/g):
150
Pore Size (Å):
140
PoreVolume (mL/g):
0.66
Hypersil
®
BDS
Pinnacle
™
DB
