5
Figure 10: PAHs onPinnacle
™
DB andHypersil
®
BDSC18 columns.
Restek Corporation • (800) 356-1688 •
Figure 9: Phenols onPinnacle
™
DB andHypersil
®
BDSC18 columns.
0
2
4
1
2
3
6
8
10
12
14
16 min.
Column:
Dimensions:
150mm x 4.6mm
Particle Size:
5µm
Pore Size:
140Å/130Å
Conditions:
Mobile Phase: 0.7%acetic acid:methanol
(60:40 v/v)
Flow:
1.0mL/min.
Temp.:
ambient
Det.:
UV@ 254nm
Sample:
Inj.:
5µL
SampleDiluent: methanol
SampleTemp.: ambient
LC_0272
0
2
4
1
2
3
6
8
10 12 14 16 18 min.
LC_0271
RetentionTimes
PeakList:
Conc.
PinnacleDB Hypersil BDS
1. phenol
0.6mg/mL
4.647
4.807
2. 2,6-dinitrophenol
1.4mg/mL
15.318
15.751
3. 3,5-dinitrophenol
1.5mg/mL
16.691
17.191
Pinnacle
™
DBC18Column
(cat.# 9414565)
Hypersil
®
BDSC18Column
(cat.# 25105-154630*)
0
2
4
6
8
10
1
2
3
4
5
6
min.
Column:
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
140Å/130Å
Conditions:
Mobile Phase:
water:acetonitrile
(25:75 v/v)
Flow:
1.0mL/min
Temp.:
ambient
Det.:
UV@ 254nm
Sample:
Inj.:
5µL
Conc.:
100µg/mL each
SampleDiluent: acetonitrile
SampleTemp.: ambient
LC_0268
0
2
4
6
8
10
1
2
3
4
5
6
min.
LC_0267
RetentionTimes
PeakList:
PinnacleDB Hypersil BDS
1. naphthalene
3.436
3.627
2. fluorene
4.611
4.902
3. phenanthrene
5.132
5.454
4. anthracene
5.574
5.941
5. pyrene
7.320
7.806
6. chrysene
9.404
10.093
Pinnacle
™
DBC18Column
(cat.# 9414565)
Hypersil
®
BDSC18Column
(cat.# 25105-154630*)
Nearly identical retention,
peak symmetry, and
efficiency!
Nearly identical retention,
peak symmetry, and
efficiency!
*Hypersil
®
catalog number; columnnot available fromRestek.
