4
Figure 8: Acidbase testmix onPinnacle
™
DB andHypersil
®
BDSC18 columns.
Restek Corporation • (800) 356-1688 •
Figure 7: Base/neutral testmix onPinnacle
™
DB andHypersil
®
BDSC18 columns.
0
1
2
3
4
5
6
7
1
2
3
4
5
6
7
8 min.
LC_0263
0
1
2
3
4
5
6
7
1
2
3
4
5
6
7
8 min.
Column:
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
140Å/130Å
Conditions:
Mobile Phase:
50mMKH
2
PO
4
, pH 3.5:methanol (35:65 v/v)
Flow:
1.0mL/min.
Temp.:
ambient
Det.:
UV@ 254nm
Sample:
Inj.:
2µL
SampleDiluent: methanol:water (50:50 v/v)
SampleTemp.:
ambient
LC_0264
Pinnacle
™
DBC18Column
(cat.# 9414565)
Hypersil
®
BDSC18Column
(cat.# 25105-154630*)
RetentionTimes
PeakList:
Conc.
PinnacleDB Hypersil BDS
1. uracil
50µg/mL
1.676
1.705
2. aniline
0.75mg/mL
2.077
2.127
3. 2-nitroaniline
0.35mg/mL
2.618
2.687
4. 2,4-nitroaniline
0.35mg/mL
2.909
2.970
5. 3,5-xylenol
2.5mg/mL
3.668
3.804
6. anisole
2.5mg/mL
4.181
4.359
7. toluene
3.5mg/mL
6.979
7.383
0
1
2
3
1
2
3
4
5
6
7
min.
Column:
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
140Å/130Å
Conditions:
Mobile Phase:
5mMKH
2
PO
4
, pH 3.5:methanol (40:60 v/v)
Flow:
1.0mL/min.
Temp.:
ambient
Det.:
UV@ 254nm
Sample:
Inj.:
2µL
SampleDiluent: methanol:water (50:50 v/v)
SampleTemp.:
ambient
LC_0260
1
2
3
1
2
3
4
5
6
7
8 min.
LC_0259
RetentionTimes
PeakList:
Conc.
PinnacleDB Hypersil BDS
1. caffeine
0.5mg/mL
2.525
2.640
2. acetylsalicylic acid
3.5mg/mL
4.088
4.363
3. benzoic acid
2.8mg/mL
6.733
7.288
Pinnacle
™
DBC18Column
(cat.# 9414565)
Hypersil
®
BDSC18Column
(cat.# 25105-154630*)
*Hypersil
®
catalog number; columnnot available fromRestek.
Nearly identical retention,
peak symmetry, and
efficiency!
Nearly identical retention,
peak symmetry, and
efficiency!
