3
Restek Corporation • (800) 356-1688 •
Figure 5: Testmix onPinnacle
™
DB andHypersil
®
BDSC18 columns.
Figure 6: Reversedphase testmix onPinnacle
™
DB andHypersil
®
BDSC18 columns.
Column:
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
140Å/130Å
Conditions:
Mobile Phase:
water:methanol (40:60 v/v)
Flow:
1.0mL/min.
Temp.:
ambient
Det.:
UV@ 254nm
Sample:
Inj.:
5µL
SampleDiluent:
methanol:water (50:50 v/v)
SampleTemp.:
ambient
0
2
4
6
1
2
3
4
8
10
min.
LC_0276
LC_0275
0
2
4
6
1
2
3
4
8
10
min.
RetentionTimes
PeakList:
Conc.
PinnacleDB Hypersil BDS
1. uracil
1µg/mL
1.666
1.737
2. pyridine
0.64mg/mL
2.155
2.196
3. N,N-dimethylaniline 0.45mg/mL
6.778
7.267
4. benzophenone
0.59mg/mL
9.006
9.646
Pinnacle
™
DBC18Column
(cat.# 9414565)
Hypersil
®
BDSC18Column
(cat.# 25105-154630*)
0
1
2
3
4
1
2
3
4
5 min.
Column:
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
140Å/130Å
Conditions:
Mobile Phase:
water:methanol (20:80 v/v)
Flow:
1.0mL/min.
Temp.:
ambient
Det.:
UV@ 254nm
Sample:
Inj.:
5µL
SampleDiluent:
methanol
SampleTemp.:
ambient
LC_0280
0
1
2
3
4
1
2
3
4
5
min.
LC_0279
Pinnacle
™
DBC18Column
(cat.# 9414565)
Hypersil
®
BDSC18Column
(cat.# 25105-154630*)
RetentionTimes
PeakList:
Conc.
PinnacleDB Hypersil BDS
1. uracil
20µg/mL
1.655
1.689
2. benzene
3mg/mL
2.546
2.791
3. naphthalene
0.5mg/mL
3.594
4.138
4. biphenyl
60µg/mL
4.529
5.396
*Hypersil
®
catalog number; columnnot available fromRestek.
Nearly identical
retention,
peak symmetry,
and efficiency!
Nearly identical
retention,
peak symmetry,
and efficiency!
