688
GC CHROMATOGRAMS |
PHARMACEUTICAL
Steroids
Steroids: Sex Hormones
Rxi®-1ms
GC_PH00872
Column:
Rxi
®
-1ms, 30m, 0.25mm ID, 0.25µm (cat.# 13323)
Sample:
100µg/mL each hormone in methanol or ethanol; compounds derivatized
using 2% methoxylamine HCl (CH
3
ONH
2
) in pyridine,
then N-trimethylsilylimidazole (TMSI), then analyzed
Inj.:
1.0µL splitless (hold 0.5 min.), 3.5mm single gooseneck inlet liner
(cat.# 20961)
Inj. temp.:
250°C
Carrier gas:
helium, constant flow
Flow rate:
1mL/min.
Oven temp.:
100°C to 320°C @ 10°C/min. (hold 10 min.)
Det:
MS: Shimadzu 17A with QP5000
Transfer line
temp.:
280°C
Scan range:
40-700amu
Ionization:
EI
Mode:
scan
1. androsterone
2. dehydroepiandrosterone (DHEA)
3. 17-
α
-estradiol
4. estrone
5. 17-
β
-estradiol
6. testosterone
7. derivatization by-product
Low bleed at 300+°C!
Excellent
resolution of
closely eluting
hormones.
Rxi® Technology!
Exceptionally inert,
ultra low- bleed
capillary columns.
Restek Customer Service
In the U.S.
Call: 800-356-1688 (ext. 3) or 814-353-1300
(ext. 3)
Monday–Friday 8:00 a.m.–6:00 p.m. ET
Fax: 814-353-1309—24-hours a day
Online:
a day
Outside the U.S.
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Refer to our list on pages 4-5 or visit our
website at
Stu Kline, Customer Service
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
