687
GC CHROMATOGRAMS |
PHARMACEUTICAL
Chi ral Analyses, Steroids
Column:
Rt
®
-
β
DEXsm, 30m, 0.32mm ID, 0.25µm
(cat.# 13104)
Inj.:
on-column, 125ng each enantiomer
Oven temp.:
175°C to 200°C @ 2°C/min.
Inj./det. temp.:
200°C/230°C
Carrier gas:
helium
Linear velocity:
60cm/sec.
Det.:
GC/FID
Split ratio:
13:1 using cup splitter inlet liner (cat.# 20709)
min.
7
8
(+/-)ibuprofen
Ibuprofen (Underivatized)
Rt®-
β
DEXsm
GC_PH00242
Column:
Rt
®
-
β
DEXcst, 30m, 0.32mm ID, 0.25µm
(cat.# 13102)
Inj.:
on-column, approximately 15ng each enantiomer
Oven temp.:
200°C to 230°C @ 1°C/min.
Inj./det. temp.:
200°C/230°C
Carrier gas:
hydrogen
Linear velocity:
80cm/sec.
Detector type:
GC/FID
Split ratio:
13:1 using cup splitter inlet liner (cat.# 20709)
min.
20
25
(+/-)thalidomide
Thalidomide (Underivatized)
Rt®-
β
DEXcst
GC_PH00243
6
Column:
Rtx
®
-5, 30m, 0.25mm ID, 0.10µm (cat.# 10208)
Sample:
0.5µL split injection of anabolic steroids
Conc.:
1,000ng/µL
Oven temp.:
180°C to 340°C @ 10°C/min. (hold 3 min.)
Inj./det. temp.:
280°C/340°C
Carrier gas:
helium
Linear velocity:
35cm/sec. set @ 180°C
FID sensitivity:
1.28 x 10
-10
AFS
Split ratio:
50:1
1. 5-androstene-3
β
,17
β
-diol
2. 17
α
-methyl-5-androstene-3
β
,17
β
-diol
3. 5
α
-androstan-17
β
-ol-3-one
4. 19-nortestosterone
5. 17
α
-methylandrostan-17
β
-ol-3-one
6. mesterolone
7. testosterone
8. 17
α
-methyltestosterone
9. 1-dehydrotestosterone
10. 1-dehydro-17
α
-methyltestosterone
11. bolasterone
12. oxymethalone
13. 19-nortestosterone-17-propionate
14. testosterone propionate
15. fluoxymesterone
16. 4-chlorotestosterone-17-acetate
17. testosterone-17
β
-cypionate
18. 1-dehydrotestosterone benzoate
19. 1-dehydrotestosterone undecylenate
Steroids, Anabolic (Underivatized)
Rtx®-5
1
19
18
17
2
3
5
7
8
9
10,11
12
13
14
15
16
4
min.
2
4
6
8
10
12
14
16
18
GC_PH00255
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
